Control of Glycolysis in Cultured Chick Embryo Hepatocytes. Fructose 2,6-bisphosphate Content and Phosphofructokinase-1 Activity Are Stimulated by Insulin and Epidermal Growth Factor

Overview

Journal Biochem J

Specialty Biochemistry

Date 1990 Aug 1

PMID 2143894

Citations 6

Authors

M J Hamer

A J Dickson

Affiliations

Soon will be listed here.

Abstract

Chick embryo hepatocytes were maintained in monolayer culture in a serum-free chemically defined medium for periods of up to 2 days. Over this time period, insulin provoked selective increases (up to 5-fold) in factors relevant to the control of glycolysis: the activities of phosphofructokinase-1 (PFK-1), phosphofructokinase-2 (PFK-2) and hexokinase isoenzymes and the content of fructose 2,6-bisphosphate (F26BP). Half-maximal effects of insulin on pFK-1 activity were in the physiological range (0.1 nM). Changes in enzyme activities and F26BP content in response to insulin were correlated with stimulation of glycolytic flux as estimated by radioisotopic flux. These data are discussed in relation to known changes which occur in hepatic glycolytic activity and PFK-1 activity in the intact chick around hatching. The effects of insulin on F26BP content, PFK-1 activity and glycolytic flux were mimicked by epidermal growth factor (EGF). In contrast, phorbol esters produced minimal actions on any of the above parameters. Our data indicate that protein kinase C is not involved in the actions of insulin or EGF in control of F26BP content or PFK-1 activity. This work indicates that the related tyrosyl kinase receptors of insulin and EGF may provoke identical responses within hepatocytes, but through the utilization of different transduction systems which merge to common control points.

Citing Articles

Variable protein homeostasis in housekeeping and non-housekeeping pathways under mycotoxins stress.

Sun Y, Wen J, Chen R, Deng Y Sci Rep. 2019; 9(1):7819.

PMID: 31127180 PMC: 6534621. DOI: 10.1038/s41598-019-44305-0.

Aegle marmelos differentially affects hepatic markers of glycolysis, insulin signalling pathway, hypoxia, and inflammation in HepG2 cells grown in fructose versus glucose-rich environment.

Aggarwal H, Nair J, Sharma P, Sehgal R, Naeem U, Rajora P Mol Cell Biochem. 2017; 438(1-2):1-16.

PMID: 28766170 DOI: 10.1007/s11010-017-3108-8.

SREBP isoform and SREBP target gene expression during rat primary hepatocyte culture.

Wu J, Dickson A In Vitro Cell Dev Biol Anim. 2010; 46(8):657-63.

PMID: 20568021 DOI: 10.1007/s11626-010-9321-3.

Dynamics of the cellular metabolome during human cytomegalovirus infection.

Munger J, Bajad S, Coller H, Shenk T, Rabinowitz J PLoS Pathog. 2006; 2(12):e132.

PMID: 17173481 PMC: 1698944. DOI: 10.1371/journal.ppat.0020132.

6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase: head-to-head with a bifunctional enzyme that controls glycolysis.

Rider M, Bertrand L, Vertommen D, Michels P, Rousseau G, Hue L Biochem J. 2004; 381(Pt 3):561-79.

PMID: 15170386 PMC: 1133864. DOI: 10.1042/BJ20040752.

References

Silpananta P, GOODRIDGE A . Synthesis and degradation of malic enzyme in chick liver. J Biol Chem. 1971; 246(18):5754-61. View

Burton K . A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956; 62(2):315-23. PMC: 1215910. DOI: 10.1042/bj0620315. View

URETA T, Reichberg S, Radojkovic J, Slebe J . Comparative studies on glucose phosphorylating isoenzymes of vertebrates. IV. Chromatographic profiles of hexokinases from the liver of several avian species. Comp Biochem Physiol B. 1973; 45(2):445-61. DOI: 10.1016/0305-0491(73)90076-x. View

GOODRIDGE A, Garay A, Silpananta P . Regulation of lipogenesis and the total activities of lipogenic enzymes in a primary culture of hepatocytes from prenatal and early postnatal chicks. J Biol Chem. 1974; 249(5):1469-75. View

Dunaway Jr G, Weber G . Effects of hormonal and nutritional changes on rates of synthesis and degradation of hepatic phosphofructokinase isozymes. Arch Biochem Biophys. 1974; 162(2):629-37. DOI: 10.1016/0003-9861(74)90225-2. View

GOODRIDGE A . Hormonal regulation of the activity of the fatty acid synthesizing system and of the malic enzyme concentration in liver cells-1,2. Fed Proc. 1975; 34(2):117-23. View

Nimmo H, Tipton K . The purification of fructose 1,6-diphosphatase from ox liver and its activation by ethylenediaminetetra-acetate. Biochem J. 1975; 145(2):323-34. PMC: 1165221. DOI: 10.1042/bj1450323. View

Langslow D . The pancreatic insulin content and its relationship to plasma glucose and free fatty acid concentrations in the embryo and neonatal chick. Br Poult Sci. 1975; 16(4):329-33. DOI: 10.1080/00071667508416196. View

Dunaway G, SEGAL H . Purification and physiological role of a peptide stabilizing factor of rat liver phosphofructokinase. J Biol Chem. 1976; 251(8):2323-9. View

10.

Ochs R, Harris R . Studies on the relationship between glycolysis, lipogenesis, gluconeogenesis, and pyruvate kinase activity of rat and chicken hepatocytes. Arch Biochem Biophys. 1978; 190(1):193-201. DOI: 10.1016/0003-9861(78)90267-9. View

11.

Hue L . The role of futile cycles in the regulation of carbohydrate metabolism in the liver. Adv Enzymol Relat Areas Mol Biol. 1981; 52:247-331. DOI: 10.1002/9780470122976.ch4. View

12.

Wals P, Katz J . Glucokinase in bird liver: a membrane bound enzyme. Biochem Biophys Res Commun. 1981; 100(4):1543-8. DOI: 10.1016/0006-291x(81)90694-x. View

13.

Picardo M, Dickson A . Hormonal regulation of glycogen metabolism in hepatocyte suspensions isolated from chicken embryos. Comp Biochem Physiol B. 1982; 71(4):689-93. DOI: 10.1016/0305-0491(82)90482-5. View

14.

Bartrons R, Hue L, Van Schaftingen E, HERS H . Hormonal control of fructose 2,6-bisphosphate concentration in isolated rat hepatocytes. Biochem J. 1983; 214(3):829-37. PMC: 1152320. DOI: 10.1042/bj2140829. View

15.

Farnararo M, Vasta V, Bruni P, DAlessandro A . The effect of insulin on Fru-2,6-P2 levels in human fibroblasts. FEBS Lett. 1984; 171(1):117-20. DOI: 10.1016/0014-5793(84)80470-6. View

16.

Bate A, Dickson A . Ketogenesis in chick embryo isolated hepatocytes. Comp Biochem Physiol B. 1984; 78(2):437-42. DOI: 10.1016/0305-0491(84)90055-5. View

17.

Morris Jr S, Winberry L, Fisch J, Back D, GOODRIDGE A . Developmental and nutritional regulation of the messenger RNAs for fatty acid synthase, malic enzyme and albumin in the livers of embryonic and newly-hatched chicks. Mol Cell Biochem. 1984; 64(1):63-8. DOI: 10.1007/BF00420929. View

18.

Bosca L, Rousseau G, Hue L . Phorbol 12-myristate 13-acetate and insulin increase the concentration of fructose 2,6-bisphosphate and stimulate glycolysis in chicken embryo fibroblasts. Proc Natl Acad Sci U S A. 1985; 82(19):6440-4. PMC: 390732. DOI: 10.1073/pnas.82.19.6440. View

19.

Weber G, Lui M, Jayaram H, Pillwein K, Natsumeda Y, Faderan M . Regulation of purine and pyrimidine metabolism by insulin and by resistance to tiazofurin. Adv Enzyme Regul. 1985; 23:81-99. DOI: 10.1016/0065-2571(85)90041-x. View

20.

Probst I . Activation of glycolysis by insulin with a sequential increase of the 6-phosphofructo-2-kinase activity, fructose-2,6-bisphosphate level and pyruvate kinase activity in cultured rat hepatocytes. Eur J Biochem. 1985; 153(2):347-53. DOI: 10.1111/j.1432-1033.1985.tb09309.x. View