Ovine Pedomics: the First Study of the Ovine Foot 16S RRNA-based Microbiome

Overview

Journal ISME J

Publisher Oxford University Press

Specialties Environmental Health
Microbiology

Date 2011 Mar 25

PMID 21430786

Citations 28

Authors

Leo A Calvo-Bado

Brian B Oakley

Scot E Dowd

Laura E Green

Graham F Medley

Atiya Ul-Hassan

Vicky Bateman

William Gaze

Luci Witcomb

Rose Grogono-Thomas

Jasmeet Kaler

Claire L Russell

Elizabeth M H Wellington

Affiliations

Soon will be listed here.

Abstract

We report the first study of the bacterial microbiome of ovine interdigital skin based on 16S rRNA by pyrosequencing and conventional cloning with Sanger-sequencing. Three flocks were selected, one a flock with no signs of footrot or interdigital dermatitis, a second flock with interdigital dermatitis alone and a third flock with both interdigital dermatitis and footrot. The sheep were classified as having either healthy interdigital skin (H) and interdigital dermatitis (ID) or virulent footrot (VFR). The ovine interdigital skin bacterial community varied significantly by flock and clinical condition. The diversity and richness of operational taxonomic units was greater in tissue from sheep with ID than H or VFR-affected sheep. Actinobacteria, Bacteriodetes, Firmicutes and Proteobacteria were the most abundant phyla comprising 25 genera. Peptostreptococcus, Corynebacterium and Staphylococcus were associated with H, ID and VFR, respectively. Sequences of Dichelobacter nodosus, the causal agent of ovine footrot, were not amplified because of mismatches in the 16S rRNA universal forward primer (27F). A specific real-time PCR assay was used to demonstrate the presence of D. nodosus, which was detected in all samples including the flock with no signs of ID or VFR. Sheep with ID had significantly higher numbers of D. nodosus (10(4)-10(9) cells per g tissue) than those with H or VFR feet.

Citing Articles

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Natural Mycoplasma Infection Reduces Expression of Pro-Inflammatory Cytokines in Response to Ovine Footrot Pathogens.

Blanchard A, Baumbach C, Michler J, Pickwell N, Staley C, Franklin J Animals (Basel). 2022; 12(23).

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Field Validation of a Non-carcinogenic and Eco-Friendly Disinfectant in a Stand-In Footbath for Treatment of Footrot Associated With -Positive Strains of in Swiss Sheep Flocks.

Schmid R, Steiner A, Becker J, Baumberger S, Durr S, Alsaaod M Front Vet Sci. 2022; 9:812638.

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Differences in composition of interdigital skin microbiota predict sheep and feet that develop footrot.

Clifton R, Monaghan E, Green M, Purdy K, Green L Sci Rep. 2022; 12(1):8931.

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Prevalence of bacterial species associated with ovine footrot and contagious ovine digital dermatitis in Swedish slaughter lambs.

Rosander A, Albinsson R, Konig U, Nyman A, Frosth S Acta Vet Scand. 2022; 64(1):6.

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