High-resolution Fourier-transform Infrared Chemical Imaging with Multiple Synchrotron Beams

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2011 Mar 23

PMID 21423192

Citations 80

Authors

Michael J Nasse

Michael J Walsh

Eric C Mattson

Ruben Reininger

Andre Kajdacsy-Balla

Virgilia Macias

Rohit Bhargava

Carol J Hirschmugl

Affiliations

Soon will be listed here.

Abstract

Conventional Fourier-transform infrared (FTIR) microspectroscopic systems are limited by an inevitable trade-off between spatial resolution, acquisition time, signal-to-noise ratio (SNR) and sample coverage. We present an FTIR imaging approach that substantially extends current capabilities by combining multiple synchrotron beams with wide-field detection. This advance allows truly diffraction-limited high-resolution imaging over the entire mid-infrared spectrum with high chemical sensitivity and fast acquisition speed while maintaining high-quality SNR.

Citing Articles

GLS2 links glutamine metabolism and atherosclerosis by remodeling artery walls.

Murcy F, Borowczyk C, Gourion-Arsiquaud S, Torrino S, Ouahrouche N, Barouillet T Nat Cardiovasc Res. 2024; 3(12):1454-1467.

PMID: 39562782 DOI: 10.1038/s44161-024-00566-1.

High-Fidelity Micro- and Nano-Scale Infrared Spectroscopic Imaging.

Bhargava R, Yeh K, Kenkel S Microsc Microanal. 2024; 29(Suppl 1):562.

PMID: 39479385 PMC: 11057005. DOI: 10.1093/micmic/ozad067.268.

Modeling the Thermoelastic Sample Response for Subdiffraction Infrared Spectroscopic Imaging.

Kenkel S, Bhargava R Chem Biomed Imaging. 2024; 2(6):413-421.

PMID: 38939874 PMC: 11200252. DOI: 10.1021/cbmi.4c00018.

Phase-shifting optothermal microscopy enables live-cell mid-infrared hyperspectral imaging of large cell populations at high confluency.

Yuan T, Riobo L, Gasparin F, Ntziachristos V, Pleitez M Sci Adv. 2024; 10(8):eadj7944.

PMID: 38381817 PMC: 10881023. DOI: 10.1126/sciadv.adj7944.

Infrared spectroscopic laser scanning confocal microscopy for whole-slide chemical imaging.

Yeh K, Sharma I, Falahkheirkhah K, Confer M, Orr A, Liu Y Nat Commun. 2023; 14(1):5215.

PMID: 37626026 PMC: 10457288. DOI: 10.1038/s41467-023-40740-w.

References

Bhargava R, Levin I . Fourier transform infrared imaging: theory and practice. Anal Chem. 2001; 73(21):5157-67. DOI: 10.1021/ac010380m. View

Fernandez D, Bhargava R, Hewitt S, Levin I . Infrared spectroscopic imaging for histopathologic recognition. Nat Biotechnol. 2005; 23(4):469-74. DOI: 10.1038/nbt1080. View

Hazen T, Dubinsky E, DeSantis T, Andersen G, Piceno Y, Singh N . Deep-sea oil plume enriches indigenous oil-degrading bacteria. Science. 2010; 330(6001):204-8. DOI: 10.1126/science.1195979. View

Holman H, Bechtel H, Hao Z, Martin M . Synchrotron IR spectromicroscopy: chemistry of living cells. Anal Chem. 2010; 82(21):8757-65. DOI: 10.1021/ac100991d. View

Prati S, Joseph E, Sciutto G, Mazzeo R . New advances in the application of FTIR microscopy and spectroscopy for the characterization of artistic materials. Acc Chem Res. 2010; 43(6):792-801. DOI: 10.1021/ar900274f. View

Kuzyk A, Kastyak M, Agrawal V, Gallant M, Sivakumar G, Rak M . Association among amyloid plaque, lipid, and creatine in hippocampus of TgCRND8 mouse model for Alzheimer disease. J Biol Chem. 2010; 285(41):31202-7. PMC: 2951194. DOI: 10.1074/jbc.M110.142174. View

Bhargava R, Perlman R, Fernandez D, Levin I, Bartick E . Non-invasive detection of superimposed latent fingerprints and inter-ridge trace evidence by infrared spectroscopic imaging. Anal Bioanal Chem. 2009; 394(8):2069-75. DOI: 10.1007/s00216-009-2817-6. View

Dumas P, Jamin N, Teillaud J, Miller L, Beccard B . Imaging capabilities of synchrotron infrared microspectroscopy. Faraday Discuss. 2004; 126:289-302. DOI: 10.1039/b305065c. View

Politi Y, Arad T, Klein E, Weiner S, Addadi L . Sea urchin spine calcite forms via a transient amorphous calcium carbonate phase. Science. 2004; 306(5699):1161-4. DOI: 10.1126/science.1102289. View

10.

Lasch P, Naumann D . Spatial resolution in infrared microspectroscopic imaging of tissues. Biochim Biophys Acta. 2006; 1758(7):814-29. DOI: 10.1016/j.bbamem.2006.06.008. View

11.

Martin F, Kelly J, Llabjani V, Martin-Hirsch P, Patel I, Trevisan J . Distinguishing cell types or populations based on the computational analysis of their infrared spectra. Nat Protoc. 2010; 5(11):1748-60. DOI: 10.1038/nprot.2010.133. View

12.

Walsh M, Fellous T, Hammiche A, Lin W, Fullwood N, Grude O . Fourier transform infrared microspectroscopy identifies symmetric PO(2)(-) modifications as a marker of the putative stem cell region of human intestinal crypts. Stem Cells. 2007; 26(1):108-18. DOI: 10.1634/stemcells.2007-0196. View

13.

Walsh M, Hammiche A, Fellous T, Nicholson J, Cotte M, Susini J . Tracking the cell hierarchy in the human intestine using biochemical signatures derived by mid-infrared microspectroscopy. Stem Cell Res. 2009; 3(1):15-27. DOI: 10.1016/j.scr.2009.02.003. View

14.

Miller L, Dumas P . Chemical imaging of biological tissue with synchrotron infrared light. Biochim Biophys Acta. 2006; 1758(7):846-57. DOI: 10.1016/j.bbamem.2006.04.010. View

15.

Kidder L, Levin I, Lewis E, Kleiman V, Heilweil E . Mercury cadmium telluride focal-plane array detection for mid-infrared Fourier-transform spectroscopic imaging. Opt Lett. 1997; 22(10):742-4. DOI: 10.1364/ol.22.000742. View

16.

Petibois C, Cestelli-Guidi M, Piccinini M, Moenner M, Marcelli A . Synchrotron radiation FTIR imaging in minutes: a first step towards real-time cell imaging. Anal Bioanal Chem. 2010; 397(6):2123-9. DOI: 10.1007/s00216-010-3817-2. View

17.

Pezacki J, Blake J, Danielson D, Kennedy D, Lyn R, Singaravelu R . Chemical contrast for imaging living systems: molecular vibrations drive CARS microscopy. Nat Chem Biol. 2011; 7(3):137-45. PMC: 7098185. DOI: 10.1038/nchembio.525. View

18.

Bhargava R . Towards a practical Fourier transform infrared chemical imaging protocol for cancer histopathology. Anal Bioanal Chem. 2007; 389(4):1155-69. DOI: 10.1007/s00216-007-1511-9. View

19.

Lewis E, Treado P, REEDER R, Story G, Dowrey A, Marcott C . Fourier transform spectroscopic imaging using an infrared focal-plane array detector. Anal Chem. 1995; 67(19):3377-81. DOI: 10.1021/ac00115a003. View

20.

Wetzel D, Levine S . Imaging molecular chemistry with infrared microscopy. Science. 1999; 285(5431):1224-5. DOI: 10.1126/science.285.5431.1224. View