Two Complex Regions, Including a TATA Sequence, Are Required for Transcription by RNA Polymerase I in Neurospora Crassa

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1990 Apr 11

PMID 2139932

Citations 3

Authors

B M Tyler

Affiliations

Soon will be listed here.

Abstract

In order to define the RNA polymerase I transcriptional apparatus and how it might interact with regulatory signals, the DNA sequences necessary for 40S rRNA transcription in Neurospora crassa were determined. A systematic set of deletion, substitution and insertion mutations were assayed in a homologous in vitro system. The sequences required for transcription of the gene consist of two large domains (I and II) from -113 to -37, and -29 to +4, respectively. Complete deletion of either domain abolished transcription. Upstream sequences confer a small stimulation of transcription. Domain II includes a TATA sequence at -5 which is sensitive to a small (2 bp) substitution and which is conserved among the large rRNA genes of many organisms. Domain I includes a sequence, termed the 'Ribo box', which is also required for transcription of the Neurospora 5S rRNA genes (1), and which occurs in the 5' region of a Neurospora ribosomal protein gene. The 5S and 40S Ribo boxes are shown to be functionally interchangeable.

Citing Articles

Nutritional and growth control of ribosomal protein mRNA and rRNA in Neurospora crassa.

Cujec T, Tyler B Nucleic Acids Res. 1996; 24(5):943-50.

PMID: 8600464 PMC: 145710. DOI: 10.1093/nar/24.5.943.

A Neurospora crassa ribosomal protein gene, homologous to yeast CRY1, contains sequences potentially coordinating its transcription with rRNA genes.

Tyler B, Harrison K Nucleic Acids Res. 1990; 18(19):5759-65.

PMID: 1977135 PMC: 332311. DOI: 10.1093/nar/18.19.5759.

Coordinate expression of ribosomal protein genes in Neurospora crassa and identification of conserved upstream sequences.

Shi Y, Tyler B Nucleic Acids Res. 1991; 19(23):6511-7.

PMID: 1836561 PMC: 329209. DOI: 10.1093/nar/19.23.6511.

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