A Neurospora Crassa Ribosomal Protein Gene, Homologous to Yeast CRY1, Contains Sequences Potentially Coordinating Its Transcription with RRNA Genes

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1990 Oct 11

PMID 1977135

Citations 13

Authors

B M Tyler

K Harrison

Affiliations

Soon will be listed here.

Abstract

We have isolated and sequenced a Neurospora crassa ribosomal protein gene (designated crp-2) strongly homologous to the rp59 gene (CRY1) of yeast and the S14 ribosomal protein gene of mammals. The inferred sequence of the crp-2 protein is more homologous (83%) to the mammalian S14 sequence than to the yeast rp59 sequence (69%). The gene has three intervening sequences (IVSs) two of which are offset 7 bp from the position of IVSs in the mammalian genes. None correspond to the position of the IVS in the yeast gene. Crp-2 was mapped by RFLP analysis to the right arm of linkage group III. The 5' region of the gene contains three copies of a sequence, the Ribo box, previously shown to be required for transcription of both 5S and 40S rRNA genes. We speculate that the Ribo box may coordinate ribosomal protein and rRNA gene transcription.

Citing Articles

Nutritional and growth control of ribosomal protein mRNA and rRNA in Neurospora crassa.

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Developmental regulation of the gene for formate dehydrogenase in Neurospora crassa.

Chow C, RajBhandary U J Bacteriol. 1993; 175(12):3703-9.

PMID: 8509325 PMC: 204785. DOI: 10.1128/jb.175.12.3703-3709.1993.

Isolation, sequencing, and characterization of crp-5, a gene encoding a Neurospora ribosomal protein.

Wang Z, Tarawneh K, Free S Curr Genet. 1993; 23(4):330-3.

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Molecular genetics of cryptopleurine resistance in Saccharomyces cerevisiae: expression of a ribosomal protein gene family.

Paulovich A, Thompson J, Larkin J, Li Z, Woolford Jr J Genetics. 1993; 135(3):719-30.

PMID: 8293976 PMC: 1205715. DOI: 10.1093/genetics/135.3.719.

The CRY1 gene in Chlamydomonas reinhardtii: structure and use as a dominant selectable marker for nuclear transformation.

Nelson J, Savereide P, Lefebvre P Mol Cell Biol. 1994; 14(6):4011-9.

PMID: 8196640 PMC: 358767. DOI: 10.1128/mcb.14.6.4011-4019.1994.

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