In Vitro Autoantibody Production by Normal Adult and Cord Blood B Cells

Overview

Journal J Clin Invest

Specialty General Medicine

Date 1990 Mar 1

PMID 2138166

Citations 5

Authors

D S Pisetsky

D F Jelinek

L M McAnally

C F Reich

P E Lipsky

Affiliations

Soon will be listed here.

Abstract

To investigate the repertoire of autoantibodies in humans, anti-DNA and rheumatoid factor (RF) production in vitro was assessed in cultures of adult peripheral blood B cells and neonatal umbilical venous blood B cells. B cells were stimulated under various culture conditions, using an immobilized monoclonal anti-CD3 antibody and adult T cells or Staphylococcus aureus (SA) in the presence or absence of adult T cells or factors derived from mitogen-stimulated adult T cells as polyclonal B cell activators. Total IgM, as well as IgM anti-DNA and RF, were assessed by ELISA. Total IgM production was induced from adult and neonatal B cells with SA plus T cell factors, as well as anti-CD3-stimulated T cells. RF was induced from adult and cord blood B cells by either mode of stimulation, whereas significant anti-DNA production was observed only when B cells were stimulated with anti-CD3-activated T cells. These results confirm the presence of B cell precursors for autoantibodies in the preimmune as well as normal adult repertoire, and indicate that the production of anti-DNA and RF appears to be regulated independently.

Citing Articles

Therapeutic effect of leflunomide on the development of experimental lupus nephritis in mice.

He C, Lu X, Yan Z, Wu M, Liu S, Yu Y Rheumatol Int. 2010; 32(3):633-8.

PMID: 21127878 DOI: 10.1007/s00296-010-1630-z.

Ovarian antibodies as detected by indirect immunofluorescence are unreliable in the diagnosis of autoimmune premature ovarian failure: a controlled evaluation.

Novosad J, Kalantaridou S, Tong Z, Nelson L BMC Womens Health. 2003; 3(1):2.

PMID: 12694633 PMC: 153539. DOI: 10.1186/1472-6874-3-2.

IL-2 enhances polyclonal IgM but not IgM-rheumatoid factor synthesis by activated human peripheral blood B cells.

Callaghan M, Whelan A, Feighery C, Bresnihan B Clin Exp Immunol. 1993; 93(2):212-7.

PMID: 8348746 PMC: 1554842. DOI: 10.1111/j.1365-2249.1993.tb07968.x.

Autoantibodies and antibacterial antibodies: from both sides now.

Watts R, Isenberg D Ann Rheum Dis. 1990; 49(12):961-5.

PMID: 2270967 PMC: 1004284. DOI: 10.1136/ard.49.12.961.

Genetic analysis of self-associating immunoglobulin G rheumatoid factors from two rheumatoid synovia implicates an antigen-driven response.

Olee T, Lu E, Huang D, Deftos M, Kozin F, Carson D J Exp Med. 1992; 175(3):831-42.

PMID: 1740665 PMC: 2119156. DOI: 10.1084/jem.175.3.831.

References

Hayward A, Lawton A . Induction of plasma cell differentiation of human fetal lymphocytes: evidence for functional immaturity of T and B cells. J Immunol. 1977; 119(4):1213-7. View

Nakamura M, Burastero S, Ueki Y, Larrick J, Notkins A, Casali P . Probing the normal and autoimmune B cell repertoire with Epstein-Barr virus. Frequency of B cells producing monoreactive high affinity autoantibodies in patients with Hashimoto's disease and systemic lupus erythematosus. J Immunol. 1988; 141(12):4165-72. View

Tosato G, Magrath I, Koski I, Dooley N, Blaese R . B cell differentiation and immunoregulatory T cell function in human cord blood lymphocytes. J Clin Invest. 1980; 66(2):383-8. PMC: 371721. DOI: 10.1172/JCI109867. View

Fong S, Tsoukas C, Frincke L, Lawrance S, Holbrook T, Vaughan J . Age-associated changes in Epstein-Barr virus-induced human lymphocyte autoantibody responses. J Immunol. 1981; 126(3):910-4. View

Yachie A, Miyawaki T, Nagaoki T, Yokoi T, Mukai M, Uwadana N . Regulation of B cell differentiation by T cell subsets defined with monoclonal OKT4 and OKT8 antibodies in human cord blood. J Immunol. 1981; 127(4):1314-7. View

Andersson U, Bird A, BRITTON B, Palacios R . Humoral and cellular immunity in humans studied at the cell level from birth to two years of age. Immunol Rev. 1981; 57:1-38. DOI: 10.1111/j.1600-065x.1981.tb00440.x. View

Hayward A . Development of lymphocyte responses and interactions in the human fetus and newborn. Immunol Rev. 1981; 57:39-60. DOI: 10.1111/j.1600-065x.1981.tb00441.x. View

Marion T, Lawton 3rd A, Kearney J, Briles D . Anti-DNA autoantibodies in (NZB X NZW)F1 mice are clonally heterogeneous, but the majority share a common idiotype. J Immunol. 1982; 128(2):668-74. View

Dziarski R . Preferential induction of autoantibody secretion in polyclonal activation by peptidoglycan and lipopolysaccharide. I. In vitro studies. J Immunol. 1982; 128(3):1018-25. View

10.

Tan E . Autoantibodies to nuclear antigens (ANA): their immunobiology and medicine. Adv Immunol. 1982; 33:167-240. DOI: 10.1016/s0065-2776(08)60836-6. View

11.

Pisetsky D, Caster S . The B-cell repertoire for autoantibodies: frequency of precursor cells for anti-DNA antibodies. Cell Immunol. 1982; 72(2):294-305. DOI: 10.1016/0008-8749(82)90477-4. View

12.

Andersson U, Britton S, De Ley M, Bird G . Evidence for the ontogenic precedence of suppressor T cell functions in the human neonate. Eur J Immunol. 1983; 13(1):6-13. DOI: 10.1002/eji.1830130104. View

13.

Welch M, Fong S, Vaughan J, Carson D . Increased frequency of rheumatoid factor precursor B lymphocytes after immunization of normal adults with tetanus toxoid. Clin Exp Immunol. 1983; 51(2):299-304. PMC: 1536905. View

14.

Dighiero G, Guilbert B, Fermand J, Lymberi P, DANON F, Avrameas S . Thirty-six human monoclonal immunoglobulins with antibody activity against cytoskeleton proteins, thyroglobulin, and native DNA: immunologic studies and clinical correlations. Blood. 1983; 62(2):264-70. View

15.

Hoch S, Schur P, Schwaber J . Frequency of anti-DNA antibody producing cells from normals and patients with systemic lupus erythematosus. Clin Immunol Immunopathol. 1983; 27(1):28-37. DOI: 10.1016/0090-1229(83)90053-3. View

16.

Cairns E, Block J, Bell D . Anti-DNA autoantibody-producing hybridomas of normal human lymphoid cell origin. J Clin Invest. 1984; 74(3):880-7. PMC: 425243. DOI: 10.1172/JCI111505. View

17.

Wakasugi N, Virelizier J . Defective IFN-gamma production in the human neonate. I. Dysregulation rather than intrinsic abnormality. J Immunol. 1985; 134(1):167-71. View

18.

Dighiero G, Lymberi P, Holmberg D, Lundquist I, Coutinho A, Avrameas S . High frequency of natural autoantibodies in normal newborn mice. J Immunol. 1985; 134(2):765-71. View

19.

Schwartz R, Stollar B . Origins of anti-DNA autoantibodies. J Clin Invest. 1985; 75(2):321-7. PMC: 423484. DOI: 10.1172/JCI111704. View

20.

Rauch J, Tannenbaum H, Straaton K, Massicotte H, Wild J . Human-human hybridoma autoantibodies with both anti-DNA and rheumatoid factor activities. J Clin Invest. 1986; 77(1):106-12. PMC: 423315. DOI: 10.1172/JCI112263. View