Differential Muscle Gene Expression As a Function of Disease Progression in Goto-Kakizaki Diabetic Rats

Overview

Journal Mol Cell Endocrinol

Specialties Cell Biology
Endocrinology
Molecular Biology

Date 2011 Mar 2

PMID 21356272

Citations 23

Authors

Jing Nie

Bai Xue

Siddharth Sukumaran

William J Jusko

Debra C DuBois

Richard R Almon

Affiliations

Soon will be listed here.

Abstract

The Goto-Kakizaki (GK) rat, a polygenic non-obese model of type 2 diabetes, is a useful surrogate for study of diabetes-related changes independent of obesity. GK rats and appropriate controls were killed at 4, 8, 12, 16 and 20 weeks post-weaning and differential muscle gene expression along with body and muscle weights, plasma hormones and lipids, and blood cell measurements were carried out. Gene expression analysis identified 204 genes showing 2-fold or greater differences between GK and controls in at least 3 ages. Array results suggested increased oxidative capacity in GK muscles, as well as differential gene expression related to insulin resistance, which was also indicated by HOMA-IR measurements. In addition, potential new biomarkers in muscle gene expression were identified that could be either a cause or consequence of T2DM. Furthermore, we demonstrate here the presence of chronic inflammation evident both systemically and in the musculature, despite the absence of obesity.

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References

Steiler T, Galuska D, Leng Y, Chibalin A, Gilbert M, Zierath J . Effect of hyperglycemia on signal transduction in skeletal muscle from diabetic Goto-Kakizaki rats. Endocrinology. 2003; 144(12):5259-67. DOI: 10.1210/en.2003-0447. View

Krook A, Kawano Y, Song X, Efendic S, Roth R, Wallberg-Henriksson H . Improved glucose tolerance restores insulin-stimulated Akt kinase activity and glucose transport in skeletal muscle from diabetic Goto-Kakizaki rats. Diabetes. 1997; 46(12):2110-4. DOI: 10.2337/diab.46.12.2110. View

Almon R, DuBois D, Yao Z, Hoffman E, Ghimbovschi S, Jusko W . Microarray analysis of the temporal response of skeletal muscle to methylprednisolone: comparative analysis of two dosing regimens. Physiol Genomics. 2007; 30(3):282-99. PMC: 4186702. DOI: 10.1152/physiolgenomics.00242.2006. View

Padilla D, McDonough P, Behnke B, Kano Y, Hageman K, Musch T . Effects of Type II diabetes on muscle microvascular oxygen pressures. Respir Physiol Neurobiol. 2006; 156(2):187-95. DOI: 10.1016/j.resp.2006.08.008. View

Goldfine I, Maddux B, Youngren J, Reaven G, Accili D, Trischitta V . The role of membrane glycoprotein plasma cell antigen 1/ectonucleotide pyrophosphatase phosphodiesterase 1 in the pathogenesis of insulin resistance and related abnormalities. Endocr Rev. 2008; 29(1):62-75. PMC: 2244935. DOI: 10.1210/er.2007-0004. View

Moxley 3rd R, Griggs R, Goldblatt D, Vangelder V, Herr B, Thiel R . Decreased insulin sensitivity of forearm muscle in myotonic dystrophy. J Clin Invest. 1978; 62(4):857-67. PMC: 371838. DOI: 10.1172/JCI109198. View

Yuan J . Modeling blood/plasma concentrations in dosed feed and dosed drinking water toxicology studies. Toxicol Appl Pharmacol. 1993; 119(1):131-41. DOI: 10.1006/taap.1993.1052. View

Bisbis S, Bailbe D, Tormo M, Picarel-Blanchot F, Derouet M, Simon J . Insulin resistance in the GK rat: decreased receptor number but normal kinase activity in liver. Am J Physiol. 1993; 265(5 Pt 1):E807-13. DOI: 10.1152/ajpendo.1993.265.5.E807. View

Bergman R, Ader M . Free fatty acids and pathogenesis of type 2 diabetes mellitus. Trends Endocrinol Metab. 2000; 11(9):351-6. DOI: 10.1016/s1043-2760(00)00323-4. View

10.

Bitar M, Al-Saleh E, Al-Mulla F . Oxidative stress--mediated alterations in glucose dynamics in a genetic animal model of type II diabetes. Life Sci. 2005; 77(20):2552-73. DOI: 10.1016/j.lfs.2005.01.033. View

11.

Abate N, Chandalia M, Satija P, Adams-Huet B, Grundy S, Sandeep S . ENPP1/PC-1 K121Q polymorphism and genetic susceptibility to type 2 diabetes. Diabetes. 2005; 54(4):1207-13. DOI: 10.2337/diabetes.54.4.1207. View

12.

Mulvey C, Harno E, Keenan A, Ohlendieck K . Expression of the skeletal muscle dystrophin-dystroglycan complex and syntrophin-nitric oxide synthase complex is severely affected in the type 2 diabetic Goto-Kakizaki rat. Eur J Cell Biol. 2005; 84(11):867-83. DOI: 10.1016/j.ejcb.2005.06.007. View

13.

Maddux B, Chang Y, Accili D, McGuinness O, Youngren J, Goldfine I . Overexpression of the insulin receptor inhibitor PC-1/ENPP1 induces insulin resistance and hyperglycemia. Am J Physiol Endocrinol Metab. 2005; 290(4):E746-9. DOI: 10.1152/ajpendo.00298.2005. View

14.

Frangioudakis G, Cooney G . Acute elevation of circulating fatty acids impairs downstream insulin signalling in rat skeletal muscle in vivo independent of effects on stress signalling. J Endocrinol. 2008; 197(2):277-85. DOI: 10.1677/JOE-07-0623. View

15.

Patti M, Butte A, Crunkhorn S, Cusi K, Berria R, Kashyap S . Coordinated reduction of genes of oxidative metabolism in humans with insulin resistance and diabetes: Potential role of PGC1 and NRF1. Proc Natl Acad Sci U S A. 2003; 100(14):8466-71. PMC: 166252. DOI: 10.1073/pnas.1032913100. View

16.

Almon R, DuBois D, Lai W, Xue B, Nie J, Jusko W . Gene expression analysis of hepatic roles in cause and development of diabetes in Goto-Kakizaki rats. J Endocrinol. 2008; 200(3):331-46. DOI: 10.1677/JOE-08-0404. View

17.

Dadke S, Li H, Kusari A, Begum N, Kusari J . Elevated expression and activity of protein-tyrosine phosphatase 1B in skeletal muscle of insulin-resistant type II diabetic Goto-Kakizaki rats. Biochem Biophys Res Commun. 2000; 274(3):583-9. DOI: 10.1006/bbrc.2000.3188. View

18.

Kanoh Y, Bandyopadhyay G, Sajan M, Standaert M, Farese R . Rosiglitazone, insulin treatment, and fasting correct defective activation of protein kinase C-zeta/lambda by insulin in vastus lateralis muscles and adipocytes of diabetic rats. Endocrinology. 2001; 142(4):1595-605. DOI: 10.1210/endo.142.4.8066. View

19.

Padilla D, McDonough P, Behnke B, Kano Y, Hageman K, Musch T . Effects of Type II diabetes on capillary hemodynamics in skeletal muscle. Am J Physiol Heart Circ Physiol. 2006; 291(5):H2439-44. DOI: 10.1152/ajpheart.00290.2006. View

20.

Yasuda K, Nishikawa W, Iwanaka N, Nakamura E, Seino Y, Tsuda K . Abnormality in fibre type distribution of soleus and plantaris muscles in non-obese diabetic Goto-Kakizaki rats. Clin Exp Pharmacol Physiol. 2002; 29(11):1001-8. DOI: 10.1046/j.1440-1681.2002.03757.x. View