PrimerProspector: De Novo Design and Taxonomic Analysis of Barcoded Polymerase Chain Reaction Primers

Overview

Journal Bioinformatics

Publisher Oxford University Press

Specialty Biology

Date 2011 Feb 26

PMID 21349862

Citations 202

Authors

William A Walters

J Gregory Caporaso

Christian L Lauber

Donna Berg-Lyons

Noah Fierer

Rob Knight

Affiliations

Soon will be listed here.

Abstract

Motivation: PCR amplification of DNA is a key preliminary step in many applications of high-throughput sequencing technologies, yet design of novel barcoded primers and taxonomic analysis of novel or existing primers remains a challenging task.

Results: PrimerProspector is an open-source software package that allows researchers to develop new primers from collections of sequences and to evaluate existing primers in the context of taxonomic data.

Availability: PrimerProspector is open-source software available at http://pprospector.sourceforge.net

Contact: rob.knight@colorado.edu

Supplementary Information: Supplementary data are available at Bioinformatics online.

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