Error-correcting Barcoded Primers for Pyrosequencing Hundreds of Samples in Multiplex

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2008 Feb 12

PMID 18264105

Citations 586

Authors

Micah Hamady

Jeffrey J Walker

J Kirk Harris

Nicholas J Gold

Rob Knight

Affiliations

Soon will be listed here.

Abstract

We constructed error-correcting DNA barcodes that allow one run of a massively parallel pyrosequencer to process up to 1,544 samples simultaneously. Using these barcodes we processed bacterial 16S rRNA gene sequences representing microbial communities in 286 environmental samples, corrected 92% of sample assignment errors, and thus characterized nearly as many 16S rRNA genes as have been sequenced to date by Sanger sequencing.

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