Isolation and Transcriptome Analysis of Adult Zebrafish Cells Enriched for Skeletal Muscle Progenitors

Overview

Journal Muscle Nerve

Date 2011 Feb 22

PMID 21337346

Citations 11

Authors

Matthew S Alexander

Genri Kawahara

Alvin T Kho

Melanie H Howell

Timothy J Pusack

Jennifer A Myers

Federica Montanaro

Leonard I Zon

Jeffrey R Guyon

Louis M Kunkel

Affiliations

Soon will be listed here.

Abstract

Introduction: Over the past 10 years, the use of zebrafish for scientific research in the area of muscle development has increased dramatically. Although several protocols exist for the isolation of adult myoblast progenitors from larger fish, no standardized protocol exists for the isolation of myogenic progenitors from adult zebrafish muscle.

Methods: Using a variant of a mammalian myoblast isolation protocol, zebrafish muscle progenitors have been isolated from the total dorsal myotome. These zebrafish myoblast progenitors can be cultured for several passages and then differentiated into multinucleated, mature myotubes.

Results: Transcriptome analysis of these cells during myogenic differentiation revealed a strong downregulation of pluripotency genes, while, conversely, showing an upregulation of myogenic signaling and structural genes.

Conclusions: Together these studies provide a simple, yet detailed method for the isolation and culture of myogenic progenitors from adult zebrafish, while further promoting their therapeutic potential for the study of muscle disease and drug screening.

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