Carbon Tube Electrodes for Electrocardiography-gated Cardiac Multimodality Imaging in Mice

Overview

Journal J Am Assoc Lab Anim Sci

Date 2011 Feb 22

PMID 21333165

Citations 2

Authors

Philippe Choquet

Christian Goetz

Gaelle Aubertin

Fabrice Hubele

Sebastien Sannie

Andre Constantinesco

Affiliations

Soon will be listed here.

Abstract

This report describes a simple design of noninvasive carbon tube electrodes that facilitates electrocardiography (ECG) in mice during cardiac multimodality preclinical imaging. Both forepaws and the left hindpaw, covered by conductive gel, of mice were placed into the openings of small carbon tubes. Cardiac ECG-gated single-photon emission CT, X-ray CT, and MRI were tested (n = 60) in 20 mice. For all applications, electrodes were used in a warmed multimodality imaging cell. A heart rate of 563 ± 48 bpm was recorded from anesthetized mice regardless of the imaging technique used, with acquisition times ranging from 1 to 2 h.

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References

Vallee J, Ivancevic M, Nguyen D, Morel D, Jaconi M . Current status of cardiac MRI in small animals. MAGMA. 2004; 17(3-6):149-56. DOI: 10.1007/s10334-004-0066-4. View

Berr S, Roy R, French B, Yang Z, Gilson W, Kramer C . Black blood gradient echo cine magnetic resonance imaging of the mouse heart. Magn Reson Med. 2005; 53(5):1074-9. DOI: 10.1002/mrm.20487. View

Choquet P, Breton E, Goetz C, Marin C, Constantinesco A . Dedicated low-field MRI in mice. Phys Med Biol. 2009; 54(17):5287-99. DOI: 10.1088/0031-9155/54/17/014. View

Van Genderingen H, Sprenger M, de Ridder J, van Rossum A . Carbon-fiber electrodes and leads for electrocardiography during MR imaging. Radiology. 1989; 171(3):872. DOI: 10.1148/radiology.171.3.2717770. View

Badea C, Fubara B, Hedlund L, Johnson G . 4-D micro-CT of the mouse heart. Mol Imaging. 2005; 4(2):110-6. DOI: 10.1162/15353500200504187. View

Goldbarg A, Hellerstein H, BRUELL J, DAROCZY A . Electrocardiogram of the normal mouse, Mus musculus: general considerations and genetic aspects. Cardiovasc Res. 1968; 2(1):93-9. DOI: 10.1093/cvr/2.1.93. View

Constantinesco A, Choquet P, Monassier L, Israel-Jost V, Mertz L . Assessment of left ventricular perfusion, volumes, and motion in mice using pinhole gated SPECT. J Nucl Med. 2005; 46(6):1005-11. View

Chin B, Metzler S, Lemaire A, Curcio A, Vemulapalli S, Greer K . Left ventricular functional assessment in mice: feasibility of high spatial and temporal resolution ECG-gated blood pool SPECT. Radiology. 2007; 245(2):440-8. DOI: 10.1148/radiol.2452061973. View

Croteau E, Benard F, Cadorette J, Gauthier M, Aliaga A, Bentourkia M . Quantitative gated PET for the assessment of left ventricular function in small animals. J Nucl Med. 2003; 44(10):1655-61. View

10.

Nahrendorf M, Badea C, Hedlund L, Figueiredo J, Sosnovik D, Johnson G . High-resolution imaging of murine myocardial infarction with delayed-enhancement cine micro-CT. Am J Physiol Heart Circ Physiol. 2007; 292(6):H3172-8. PMC: 2680216. DOI: 10.1152/ajpheart.01307.2006. View

11.

Wehrens X, Kirchhoff S, Doevendans P . Mouse electrocardiography: an interval of thirty years. Cardiovasc Res. 2000; 45(1):231-7. DOI: 10.1016/s0008-6363(99)00335-1. View

12.

Wiesmann F, Ruff J, Hiller K, Rommel E, Haase A, Neubauer S . Developmental changes of cardiac function and mass assessed with MRI in neonatal, juvenile, and adult mice. Am J Physiol Heart Circ Physiol. 2000; 278(2):H652-7. DOI: 10.1152/ajpheart.2000.278.2.H652. View

13.

Feintuch A, Zhu Y, Bishop J, Davidson L, Dazai J, Bruneau B . 4D cardiac MRI in the mouse. NMR Biomed. 2007; 20(3):360-5. DOI: 10.1002/nbm.1164. View

14.

Kreissl M, Wu H, Stout D, Ladno W, Schindler T, Zhang X . Noninvasive measurement of cardiovascular function in mice with high-temporal-resolution small-animal PET. J Nucl Med. 2006; 47(6):974-80. PMC: 4348007. View

15.

Goetz C, Monassier L, Choquet P, Constantinesco A . Assessment of right and left ventricular function in healthy mice by blood-pool pinhole gated SPECT. C R Biol. 2008; 331(9):637-47. DOI: 10.1016/j.crvi.2008.06.001. View

16.

Gilson W, Kraitchman D . Cardiac magnetic resonance imaging in small rodents using clinical 1.5 T and 3.0 T scanners. Methods. 2007; 43(1):35-45. PMC: 2075472. DOI: 10.1016/j.ymeth.2007.03.012. View

17.

Tsui B, Kraitchman D . Recent advances in small-animal cardiovascular imaging. J Nucl Med. 2009; 50(5):667-70. PMC: 2866288. DOI: 10.2967/jnumed.108.058479. View

18.

Franco F, Dubois S, Peshock R, Shohet R . Magnetic resonance imaging accurately estimates LV mass in a transgenic mouse model of cardiac hypertrophy. Am J Physiol. 1998; 274(2):H679-83. DOI: 10.1152/ajpheart.1998.274.2.H679. View

19.

Lahoutte T . Monitoring left ventricular function in small animals. J Nucl Cardiol. 2007; 14(3):371-9. DOI: 10.1016/j.nuclcard.2007.04.014. View

20.

Wendt 3rd R, Rokey R, Vick 3rd G, Johnston D . Electrocardiographic gating and monitoring in NMR imaging. Magn Reson Imaging. 1988; 6(1):89-95. DOI: 10.1016/0730-725x(88)90528-0. View