Effect of Adrenalectomy on Acceleration of Gluconeogenesis by Calcium Ions, Adenosine 3':5'-cyclic Monophosphate and Adrenaline in Rat Kidney Tubules

Overview

Journal Biochem J

Specialty Biochemistry

Date 1978 Aug 15

PMID 213058

Citations 2

Authors

D W Macdonald

E D Saggerson

Affiliations

Soon will be listed here.

Abstract

1. Gluconeogenesis from pyruvate was measured in renal-cortical-tubules fragments prepared from fed male rats 6-8 days after adrenalectomy or sham adrenalectomy. The response of this process to 3':5'-cyclic AMP and adrenaline was compared in these two states at two Ca2+ concentrations. 2. Adrenalectomy decreased the percentage stimulation of gluconeogenesis by 3':5'-cyclic AMP, but increased percentage stimulation by adrenaline. Cortisol treatment of adrenalectomized rats (50 mg/kg, twice daily for 2 days) did not reverse the change in responsiveness to 3':5'-cyclic AMP and adrenaline. 3. Stimulation of gluconeogenesis by 1 micron-adrenaline was unaffected by 10 micron-propranolol (beta-blocker) in either state. Phentolamine (alpha-blocker; 10 micron) totally blocked stimulation of gluconeogenesis by 1 micron-adrenaline in the sham-operated condition, but was only partially effective in this respect after adrenalectomy.

Citing Articles

Effect of compound D-600 (methoxyverapamil) on gluconeogenesis and on acceleration of the process by alpha-adrenergic stimuli in rat kidney tubules.

Saggerson E, Carpenter C Biochem J. 1980; 190(2):283-91.

PMID: 6258565 PMC: 1162092. DOI: 10.1042/bj1900283.

Evidence that catecholamines stimulate renal gluconeogenesis through an alpha 1-type of adrenoceptor.

Kessar P, Saggerson E Biochem J. 1980; 190(1):119-23.

PMID: 6255939 PMC: 1162069. DOI: 10.1042/bj1900119.

References

Burton K . A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956; 62(2):315-23. PMC: 1215910. DOI: 10.1042/bj0620315. View

Rutman J, MELTZER L, KITCHELL J, Rutman R, George P . EFFECT OF METAL IONS ON IN VITRO GLUCONEOGENESIS IN RAT KIDNEY CORTEX SLICES. Am J Physiol. 1965; 208:842-6. DOI: 10.1152/ajplegacy.1965.208.5.841. View

KREBS H, Bennett D, DE GASQUET P, GASQUET P, GASCOYNE T, Yoshida T . Renal gluconeogenesis. The effect of diet on the gluconeogenic capacity of rat-kidney-cortex slices. Biochem J. 1963; 86:22-7. PMC: 1201705. DOI: 10.1042/bj0860022. View

Macdonald D, Saggerson E . Hormonal control of gluconeogenesis in tubule fragments from renal cortex of fed rats. Effects of alpha-adrenergic stimuli, glucagon, theophylline and papaverine. Biochem J. 1977; 168(1):33-42. PMC: 1184114. DOI: 10.1042/bj1680033. View

ALLEYNE G, Flores H, Roobol A . The interrelationship of the concentration of hydrogen ions, bicarbonate ions, carbon dioxide and calcium ions in the regulation of renal gluconeogenesis in the rat. Biochem J. 1973; 136(3):445-53. PMC: 1165979. DOI: 10.1042/bj1360445. View

EXTON J, Friedmann N, Wong E, Brineaux J, Corbin J, Park C . Interaction of glucocorticoids with glucagon and epinephrine in the control of gluconeogenesis and glycogenolysis in liver and of lipolysis in adipose tissue. J Biol Chem. 1972; 247(11):3579-88. View

Nagata N, Rasmussen H . Renal gluconeogenesis: effects of Ca2+ and H+. Biochim Biophys Acta. 1970; 215(1):1-16. DOI: 10.1016/0304-4165(70)90382-x. View

Roobol A, ALLEYNE G . Regulation of renal gluconeogenesis by calcium ions, hormones and adenosine 3':5'-cyclic monophosphate. Biochem J. 1973; 134(1):157-65. PMC: 1177796. DOI: 10.1042/bj1340157. View

ALLEN D, BECK R . Alterations in lipolysis, adenylate cyclase and adenosine 3', 5'-monophosphate levels in isolated fat cells following adrenalectomy. Endocrinology. 1972; 91(2):504-10. DOI: 10.1210/endo-91-2-504. View

10.

Saggerson E . The regulation of glyceride synthesis in isolated white-fat cells. The effects of palmitate and lipolytic agents. Biochem J. 1972; 128(5):1057-67. PMC: 1173993. DOI: 10.1042/bj1281057. View

11.

Guder W, Wiesner W, Stukowski B, Wieland O . Metabolism of isolated kidney tubules. Oxygen consumption, gluconeogenesis and the effect of cyclic nucleotides in tubules from starved rats. Hoppe Seylers Z Physiol Chem. 1971; 352(10):1319-28. DOI: 10.1515/bchm2.1971.352.2.1319. View

12.

Schaeffer L, Chenoweth M, Dunn A . Adrenal corticosteroid involvement in the control of liver glycogen phosphorylase activity. Biochim Biophys Acta. 1969; 192(2):292-303. DOI: 10.1016/0304-4165(69)90368-7. View

13.

Miller T, EXTON J, Park C . A block in epinephrine-induced glycogenolysis in hearts from adrenalectomized rats. J Biol Chem. 1971; 246(11):3672-8. View

14.

EXTON J, Harper S . Role of cyclic AMP in the actions of catecholamines on hepatic carbohydrate metabolism. Adv Cyclic Nucleotide Res. 1975; 5:519-32. View

15.

Saitoh Y, Ui M . Activation and inactivation of phosphorylase and glycogen synthetase during perfusion of rat liver as influenced by epinephrine, glucagon and hydrocortisone. Biochim Biophys Acta. 1975; 404(1):7-17. DOI: 10.1016/0304-4165(75)90142-7. View

16.

Guder W, Rupprecht A . Metabolism of isolated kidney tubules. Independent actions of catecholamines on renal cyclic adenosine 3':5'-monophosphate levels and gluconeogenesis. Eur J Biochem. 1975; 52(2):283-90. DOI: 10.1111/j.1432-1033.1975.tb03996.x. View

17.

Chen R . Removal of fatty acids from serum albumin by charcoal treatment. J Biol Chem. 1967; 242(2):173-81. View