A Functionally Characterized Test Set of Human Induced Pluripotent Stem Cells

Overview

Journal Nat Biotechnol

Specialty Biotechnology

Date 2011 Feb 5

PMID 21293464

Citations 264

Authors

Gabriella L Boulting

Evangelos Kiskinis

Gist F Croft

Mackenzie W Amoroso

Derek H Oakley

Brian J Wainger

Damian J Williams

David J Kahler

Mariko Yamaki

Lance Davidow

Christopher T Rodolfa

John T Dimos

Shravani Mikkilineni

Amy B MacDermott

Clifford J Woolf

Christopher E Henderson

Hynek Wichterle

Kevin Eggan

Affiliations

Soon will be listed here.

Abstract

Human induced pluripotent stem cells (iPSCs) present exciting opportunities for studying development and for in vitro disease modeling. However, reported variability in the behavior of iPSCs has called their utility into question. We established a test set of 16 iPSC lines from seven individuals of varying age, sex and health status, and extensively characterized the lines with respect to pluripotency and the ability to terminally differentiate. Under standardized procedures in two independent laboratories, 13 of the iPSC lines gave rise to functional motor neurons with a range of efficiencies similar to that of human embryonic stem cells (ESCs). Although three iPSC lines were resistant to neural differentiation, early neuralization rescued their performance. Therefore, all 16 iPSC lines passed a stringent test of differentiation capacity despite variations in karyotype and in the expression of early pluripotency markers and transgenes. This iPSC and ESC test set is a robust resource for those interested in the basic biology of stem cells and their applications.

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