A Rearranged EP300 Gene in the Human B-cell Lymphoma Cell Line RC-K8 Encodes a Disabled Transcriptional Co-activator That Contributes to Cell Growth and Oncogenicity

Overview

Journal Cancer Lett

Specialty Oncology

Date 2011 Jan 15

PMID 21232847

Citations 9

Authors

Michael R Garbati

Ryan C Thompson

Leila Haery

Thomas D Gilmore

Affiliations

Soon will be listed here.

Abstract

Human diffuse large B-cell lymphoma cell line RC-K8 has an altered EP300 locus that encodes a C-terminally truncated histone acetyltransferase (HAT) protein (p300ΔC). We now show that p300ΔC contains 1047N-terminal amino acids of p300 fused to 25 amino acids encoded by sequences from chromosome 6. Over-expressed p300ΔC localized to nuclear subdomains and interacted with transcription factor REL. p300ΔC did not function as a co-activator for REL-directed transactivation, and blocked the ability of wild-type p300 to enhance transcriptional activation by REL. Knock down of p300ΔC in RC-K8 cells reduced their growth in both liquid culture and soft agar. Truncations of p300 were not found in eight other B-lymphoma cell lines. These results suggest that p300ΔC contributes to the oncogenic state of RC-K8 cells by acting as a defective co-activator.

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