» Articles » PMID: 21221806

Musashi1 and Hairy and Enhancer of Split 1 High Expression Cells Derived from Embryonic Stem Cells Enhance the Repair of Small-intestinal Injury in the Mouse

Overview
Journal Dig Dis Sci
Specialty Gastroenterology
Date 2011 Jan 12
PMID 21221806
Citations 3
Authors
Affiliations
Soon will be listed here.
Abstract

Background: Embryonic stem cells have great plasticity. In this study, we repaired impaired small intestine by transplanting putative intestinal epithelial stem cells (Musashi1 and hairy and enhancer of split 1 high expression cells) derived from embryonic stem cells.

Methods: The differentiation of definitive endoderm in embryoid bodies, derived from male ES-E14TG2a cells by the hanging-drop method, was monitored to define a time point for maximal induction of putative intestinal epithelial stem cells by epidermal growth factor. Furthermore, to evaluate the regenerative potential of intestinal epithelium, these putative stem cells were engrafted into NOD/SCID mice and female mice with enteritis. Donor cells were located by SRY DNA in situ hybridization.

Results: The results revealed that definitive endodermal markers were highly expressed in 5-day embryoid bodies. These embryoid body cells were induced into putative intestinal epithelial stem cells on the 5th day of epidermal growth factor administration. Grafts from these cells consisted of adenoid structures and nonspecific structural cells with strong expression of small-intestinal epithelial cell markers. In situ hybridization revealed that the donor cells could specifically locate in damaged intestinal epithelium, contribute to epithelial structures, and enhance regeneration.

Conclusions: In conclusion, the Musashi1 and hairy and enhancer of split 1 high expression cells, derived from mouse embryonic stem cells, locate predominantly in impaired small-intestinal epithelium after transplantation and contribute to epithelial regeneration.

Citing Articles

Bacterial translocation in acute lymphocytic leukemia.

Song Y, Gyarmati P PLoS One. 2019; 14(4):e0214526.

PMID: 30934014 PMC: 6443231. DOI: 10.1371/journal.pone.0214526.


The RNA-Binding Protein Musashi1: A Major Player in Intestinal Epithelium Renewal and Colon Cancer Development.

Plateroti M, de Araujo P, da Silva A, Penalva L Curr Colorectal Cancer Rep. 2013; 8(4):290-297.

PMID: 23914149 PMC: 3728701. DOI: 10.1007/s11888-012-0141-6.


Musashi1 expression cells derived from mouse embryonic stem cells can be enriched in side population isolated by fluorescence activated cell sorter.

Yu T, Zhao L, Lan S, Fan M, Gong Y, Shi L BMC Cell Biol. 2011; 12:47.

PMID: 22026428 PMC: 3260164. DOI: 10.1186/1471-2121-12-47.

References
1.
Montgomery R, Breault D . Small intestinal stem cell markers. J Anat. 2008; 213(1):52-8. PMC: 2475558. DOI: 10.1111/j.1469-7580.2008.00925.x. View

2.
Wang X, Yin Y, Yuan H, Sakamaki T, Okano H, Glazer R . Musashi1 modulates mammary progenitor cell expansion through proliferin-mediated activation of the Wnt and Notch pathways. Mol Cell Biol. 2008; 28(11):3589-99. PMC: 2423292. DOI: 10.1128/MCB.00040-08. View

3.
Orlic D, Kajstura J, Chimenti S, Jakoniuk I, Anderson S, Li B . Bone marrow cells regenerate infarcted myocardium. Nature. 2001; 410(6829):701-5. DOI: 10.1038/35070587. View

4.
Abud H, Watson N, Heath J . Growth of intestinal epithelium in organ culture is dependent on EGF signalling. Exp Cell Res. 2005; 303(2):252-62. DOI: 10.1016/j.yexcr.2004.10.006. View

5.
Zhu L, Gibson P, Currle D, Tong Y, Richardson R, Bayazitov I . Prominin 1 marks intestinal stem cells that are susceptible to neoplastic transformation. Nature. 2008; 457(7229):603-7. PMC: 2633030. DOI: 10.1038/nature07589. View