Nucleotide Sequence of the Pseudomonas Fluorescens Signal Peptidase II Gene (lsp) and Flanking Genes

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1990 Nov 1

PMID 2121716

Citations 13

Authors

L Isaki

R Beers

H C Wu

Affiliations

Soon will be listed here.

Abstract

The lsp gene encoding prolipoprotein signal peptidase (signal peptidase II) is organized into an operon consisting of ileS and three open reading frames, designated genes x, orf149, and orf316 in both Escherichia coli and Enterobacter aerogenes. A plasmid, pBROC128, containing a 5.8-kb fragment of Pseudomonas fluorescens DNA was found to confer pseudomonic acid resistance on E. coli host cells and to contain the structural gene of ileS from P. fluorescens. In addition, E. coli strains carrying pBROC128 exhibited increased globomycin resistance. This indicated that the P. fluorescens lsp gene was present on the plasmid. The nucleotide sequences of the P. fluorescens lsp gene and of its flanking regions were determined. Comparison of the nucleotide sequences of the lsp genes in E. coli and P. fluorescens revealed two highly conserved domains in this enzyme. Furthermore, the five genes which constitute an operon in E. coli and Enterobacter aerogenes were found in P. fluorescens in the same order as in the first two species.

Citing Articles

Transposition of DEH, a broad-host-range transposon flanked by ISPpu12, in Pseudomonas putida is associated with genomic rearrangements and dehalogenase gene silencing.

Weightman A, Topping A, Hill K, Lee L, Sakai K, Slater J J Bacteriol. 2002; 184(23):6581-91.

PMID: 12426347 PMC: 135415. DOI: 10.1128/JB.184.23.6581-6591.2002.

Cloning and functional analysis of the pbr lead resistance determinant of Ralstonia metallidurans CH34.

Borremans B, Hobman J, Provoost A, Brown N, van der Lelie D J Bacteriol. 2001; 183(19):5651-8.

PMID: 11544228 PMC: 95457. DOI: 10.1128/JB.183.19.5651-5658.2001.

A nonessential signal peptidase II (Lsp) of Myxococcus xanthus might be involved in biosynthesis of the polyketide antibiotic TA.

Paitan Y, Orr E, Ron E, Rosenberg E J Bacteriol. 1999; 181(18):5644-51.

PMID: 10482504 PMC: 94083. DOI: 10.1128/JB.181.18.5644-5651.1999.

Occurrence of homologs of the Escherichia coli lytB gene in gram-negative bacterial species.

Potter S, Yang X, Boulanger M, Ishiguro E J Bacteriol. 1998; 180(7):1959-61.

PMID: 9537400 PMC: 107115. DOI: 10.1128/JB.180.7.1959-1961.1998.

Biochemical and genetic characterization of an FK506-sensitive peptidyl prolyl cis-trans isomerase from a thermophilic archaeon, Methanococcus thermolithotrophicus.

Furutani M, Iida T, Yamano S, Kamino K, Maruyama T J Bacteriol. 1998; 180(2):388-94.

PMID: 9440528 PMC: 106894. DOI: 10.1128/JB.180.2.388-394.1998.

References

ISAKSSON L, Skold S, Skjoldebrand J, Takata R . A procedure for isolation of spontaneous mutants with temperature sensitive of RNA and/or protein. Mol Gen Genet. 1977; 156(3):233-7. DOI: 10.1007/BF00267177. View

Isaki L, Kawakami M, Beers R, Hom R, Wu H . Cloning and nucleotide sequence of the Enterobacter aerogenes signal peptidase II (lsp) gene. J Bacteriol. 1990; 172(1):469-72. PMC: 208454. DOI: 10.1128/jb.172.1.469-472.1990. View

Nakamura K, Pirtle R, Inouye M . Homology of the gene coding for outer membrane lipoprotein within various Gram-negative bacteria. J Bacteriol. 1979; 137(1):595-604. PMC: 218487. DOI: 10.1128/jb.137.1.595-604.1979. View

Mizuno T, Kageyama M . Isolation of characterization of a major outer membrane protein of Pseudomonas aeruginosa. Evidence for the occurrence of a lipoprotein. J Biochem. 1979; 85(1):115-22. DOI: 10.1093/oxfordjournals.jbchem.a132300. View

Mizuno T . A novel peptidoglycan-associated lipoprotein found in the cell envelope of Pseudomonas aeruginosa and Escherichia coli. J Biochem. 1979; 86(4):991-1000. DOI: 10.1093/oxfordjournals.jbchem.a132631. View

Twigg A, Sherratt D . Trans-complementable copy-number mutants of plasmid ColE1. Nature. 1980; 283(5743):216-8. DOI: 10.1038/283216a0. View

Hughes J, Mellows G, Soughton S . How does Pseudomonas fluorescens, the producing organism of the antibiotic pseudomonic acid A, avoid suicide?. FEBS Lett. 1980; 122(2):322-4. DOI: 10.1016/0014-5793(80)80465-0. View

Tokunaga M, Tokunaga H, Wu H . Post-translational modification and processing of Escherichia coli prolipoprotein in vitro. Proc Natl Acad Sci U S A. 1982; 79(7):2255-9. PMC: 346170. DOI: 10.1073/pnas.79.7.2255. View

KYTE J, Doolittle R . A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982; 157(1):105-32. DOI: 10.1016/0022-2836(82)90515-0. View

10.

Messing J . New M13 vectors for cloning. Methods Enzymol. 1983; 101:20-78. DOI: 10.1016/0076-6879(83)01005-8. View

11.

Tokunaga M, Loranger J, Wu H . Isolation and characterization of an Escherichia coli clone overproducing prolipoprotein signal peptidase. J Biol Chem. 1983; 258(20):12102-5. View

12.

Devereux J, Haeberli P, SMITHIES O . A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984; 12(1 Pt 1):387-95. PMC: 321012. DOI: 10.1093/nar/12.1part1.387. View

13.

Mulligan M, HAWLEY D, Entriken R, McClure W . Escherichia coli promoter sequences predict in vitro RNA polymerase selectivity. Nucleic Acids Res. 1984; 12(1 Pt 2):789-800. PMC: 321093. DOI: 10.1093/nar/12.1part2.789. View

14.

Regue M, Remenick J, Tokunaga M, Mackie G, Wu H . Mapping of the lipoprotein signal peptidase gene (lsp). J Bacteriol. 1984; 158(2):632-5. PMC: 215476. DOI: 10.1128/jb.158.2.632-635.1984. View

15.

Tokunaga M, Loranger J, Wu H . A distinct signal peptidase for prolipoprotein in Escherichia coli. J Cell Biochem. 1984; 24(2):113-20. DOI: 10.1002/jcb.240240203. View

16.

Innis M, Tokunaga M, Williams M, Loranger J, Chang S, Chang S . Nucleotide sequence of the Escherichia coli prolipoprotein signal peptidase (lsp) gene. Proc Natl Acad Sci U S A. 1984; 81(12):3708-12. PMC: 345288. DOI: 10.1073/pnas.81.12.3708. View

17.

Yu F, Yamada H, Daishima K, Mizushima S . Nucleotide sequence of the lspA gene, the structural gene for lipoprotein signal peptidase of Escherichia coli. FEBS Lett. 1984; 173(1):264-8. DOI: 10.1016/0014-5793(84)81060-1. View

18.

Vieira J, Messing J . Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985; 33(1):103-19. DOI: 10.1016/0378-1119(85)90120-9. View

19.

Kamio Y, Lin C, Regue M, Wu H . Characterization of the ileS-lsp operon in Escherichia coli. Identification of an open reading frame upstream of the ileS gene and potential promoter(s) for the ileS-lsp operon. J Biol Chem. 1985; 260(9):5616-20. View

20.

Wu H, Tokunaga M . Biogenesis of lipoproteins in bacteria. Curr Top Microbiol Immunol. 1986; 125:127-57. DOI: 10.1007/978-3-642-71251-7_9. View