Male Infertility-linked Point Mutation Disrupts the Ca2+ Oscillation-inducing and PIP(2) Hydrolysis Activity of Sperm PLCζ

Overview

Journal Biochem J

Specialty Biochemistry

Date 2011 Jan 6

PMID 21204786

Citations 24

Authors

Michail Nomikos

Khalil Elgmati

Maria Theodoridou

Brian L Calver

Bevan Cumbes

George Nounesis

Georg Nounesis

Karl Swann

F Anthony Lai

Affiliations

Soon will be listed here.

Abstract

A male infertility-linked human PLCζ (phospholipase Cζ) mutation introduced into mouse PLCζ completely abolishes both in vitro PIP(2) (phosphatidylinositol 4,5-bisphosphate) hydrolysis activity and the ability to trigger in vivo Ca2+ oscillations in mouse eggs. Wild-type PLCζ initiated a normal pattern of Ca2+ oscillations in eggs in the presence of 10-fold higher mutant PLCζ, suggesting that infertility is not mediated by a dominant-negative mechanism.

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References

Swann K, Saunders C, Rogers N, Lai F . PLCzeta(zeta): a sperm protein that triggers Ca2+ oscillations and egg activation in mammals. Semin Cell Dev Biol. 2006; 17(2):264-73. DOI: 10.1016/j.semcdb.2006.03.009. View

Kouchi Z, Fukami K, Shikano T, Oda S, Nakamura Y, Takenawa T . Recombinant phospholipase Czeta has high Ca2+ sensitivity and induces Ca2+ oscillations in mouse eggs. J Biol Chem. 2004; 279(11):10408-12. DOI: 10.1074/jbc.M313801200. View

Schultz R, Kopf G . Molecular basis of mammalian egg activation. Curr Top Dev Biol. 1995; 30:21-62. DOI: 10.1016/s0070-2153(08)60563-3. View

Nasr-Esfahani M, Deemeh M, Tavalaee M . Artificial oocyte activation and intracytoplasmic sperm injection. Fertil Steril. 2009; 94(2):520-6. DOI: 10.1016/j.fertnstert.2009.03.061. View

Summers M, McGinnis L, Lawitts J, Raffin M, Biggers J . IVF of mouse ova in a simplex optimized medium supplemented with amino acids. Hum Reprod. 2000; 15(8):1791-801. DOI: 10.1093/humrep/15.8.1791. View

Knott J, Kurokawa M, Fissore R, Schultz R, Williams C . Transgenic RNA interference reveals role for mouse sperm phospholipase Czeta in triggering Ca2+ oscillations during fertilization. Biol Reprod. 2004; 72(4):992-6. DOI: 10.1095/biolreprod.104.036244. View

Suh P, Park J, Manzoli L, Cocco L, Peak J, Katan M . Multiple roles of phosphoinositide-specific phospholipase C isozymes. BMB Rep. 2008; 41(6):415-34. DOI: 10.5483/bmbrep.2008.41.6.415. View

Cox L, Larman M, Saunders C, Hashimoto K, Swann K, Lai F . Sperm phospholipase Czeta from humans and cynomolgus monkeys triggers Ca2+ oscillations, activation and development of mouse oocytes. Reproduction. 2002; 124(5):611-23. DOI: 10.1530/rep.0.1240611. View

Nomikos M, Blayney L, Larman M, Campbell K, Rossbach A, Saunders C . Role of phospholipase C-zeta domains in Ca2+-dependent phosphatidylinositol 4,5-bisphosphate hydrolysis and cytoplasmic Ca2+ oscillations. J Biol Chem. 2005; 280(35):31011-8. DOI: 10.1074/jbc.M500629200. View

10.

Swann K . A cytosolic sperm factor stimulates repetitive calcium increases and mimics fertilization in hamster eggs. Development. 1990; 110(4):1295-302. DOI: 10.1242/dev.110.4.1295. View

11.

Saunders C, Swann K, Lai F . PLCzeta, a sperm-specific PLC and its potential role in fertilization. Biochem Soc Symp. 2007; (74):23-36. DOI: 10.1042/BSS0740023. View

12.

Saunders C, Larman M, Parrington J, Cox L, Royse J, Blayney L . PLC zeta: a sperm-specific trigger of Ca(2+) oscillations in eggs and embryo development. Development. 2002; 129(15):3533-44. DOI: 10.1242/dev.129.15.3533. View

13.

Stricker S . Comparative biology of calcium signaling during fertilization and egg activation in animals. Dev Biol. 1999; 211(2):157-76. DOI: 10.1006/dbio.1999.9340. View

14.

Jones K . Ca2+ oscillations in the activation of the egg and development of the embryo in mammals. Int J Dev Biol. 1998; 42(1):1-10. View

15.

Ellis M, James S, Perisic O, Downes C, Williams R, Katan M . Catalytic domain of phosphoinositide-specific phospholipase C (PLC). Mutational analysis of residues within the active site and hydrophobic ridge of plcdelta1. J Biol Chem. 1998; 273(19):11650-9. DOI: 10.1074/jbc.273.19.11650. View

16.

Heytens E, Parrington J, Coward K, Young C, Lambrecht S, Yoon S . Reduced amounts and abnormal forms of phospholipase C zeta (PLCzeta) in spermatozoa from infertile men. Hum Reprod. 2009; 24(10):2417-28. DOI: 10.1093/humrep/dep207. View

17.

Yoon S, Jellerette T, Salicioni A, Lee H, Yoo M, Coward K . Human sperm devoid of PLC, zeta 1 fail to induce Ca(2+) release and are unable to initiate the first step of embryo development. J Clin Invest. 2008; 118(11):3671-81. PMC: 2567839. DOI: 10.1172/JCI36942. View

18.

Runft L, Jaffe L, Mehlmann L . Egg activation at fertilization: where it all begins. Dev Biol. 2002; 245(2):237-54. DOI: 10.1006/dbio.2002.0600. View

19.

Heindryckx B, De Gheselle S, Gerris J, Dhont M, De Sutter P . Efficiency of assisted oocyte activation as a solution for failed intracytoplasmic sperm injection. Reprod Biomed Online. 2008; 17(5):662-8. DOI: 10.1016/s1472-6483(10)60313-6. View