Quantification of in Vivo Fluorescence Decoupled from the Effects of Tissue Optical Properties Using Fiber-optic Spectroscopy Measurements

Overview

Journal J Biomed Opt

Specialties Biomedical Engineering
Ophthalmology

Date 2011 Jan 5

PMID 21198210

Citations 81

Authors

Anthony Kim

Mamta Khurana

Yumi Moriyama

Brian C Wilson

Affiliations

Soon will be listed here.

Abstract

We present a method for tissue fluorescence quantification in situ using a handheld fiber optic probe that measures both the fluorescence and diffuse reflectance spectra. A simplified method to decouple the fluorescence spectrum from distorting effects of the tissue optical absorption and scattering is developed, with the objective of accurately quantifying the fluorescence in absolute units. The primary motivation is measurement of 5-aminolevulinic acid-induced protoporphyrin IX (ALA-PpIX) concentration in tissue during fluorescence-guided resection of malignant brain tumors. This technique is validated in phantoms and ex vivo mouse tissues, and tested in vivo in a rabbit brain tumor model using ALA-PpIX fluorescence contrast.

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