Requirement for CD4+ Cells in Resistance to Pneumocystis Carinii Pneumonia in Mice

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1990 Sep 1

PMID 2117637

Citations 97

Authors

A G Harmsen

M Stankiewicz

Affiliations

Soon will be listed here.

Abstract

The importance of CD4+ cells in resistance to Pneumocystis carinii (PC) in PC-susceptible severe combined immunodeficient (SCID) mice that were made resistant to PC by immunocompetent spleen cell transfer, and in conventional PC-resistant mice, was investigated. SCID mice with naturally acquired PC pneumonia (PCP) were given infusions of spleen cells from immunocompetent donors. This reconstitution caused the recipients to resolve their PCP. Treatment of reconstituted SCID mice with anti-CD4 monoclonal antibodies (mAbs) to deplete them of CD4+ cells eliminated their ability to resolve PCP, whereas treating them with anti-CD8 mAb to deplete CD8+ cells had no effect. The findings indicate, therefore, that resistance to PCP in immunologically reconstituted SCID mice is dependent on CD4+ cells. To determine whether CD4+ cells enable conventional mice to resist PCP, B6D2 mice were treated with anti-CD4 mAb to deplete them of CD4+ cells in an attempt to induce PCP. After 10-11 wk of treatment, these mice developed progressive PCP. Taken together, these results indicate that loss of CD4+ cells predisposes mice to PC infection.

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References

Masur H, Jones T . The interaction in vitro of Pneumocystis carinii with macrophages and L-cells. J Exp Med. 1978; 147(1):157-70. PMC: 2184105. DOI: 10.1084/jem.147.1.157. View

Shellito J, Suzara V, BLUMENFELD W, Beck J, Steger H, Ermak T . A new model of Pneumocystis carinii infection in mice selectively depleted of helper T lymphocytes. J Clin Invest. 1990; 85(5):1686-93. PMC: 296622. DOI: 10.1172/JCI114621. View

Bice D, Harris D, Hill J, Muggenburg B, Wolff R . Immune responses after localized lung immunization in the dog. Am Rev Respir Dis. 1980; 122(5):755-60. DOI: 10.1164/arrd.1980.122.5.755. View

KALTREIDER H, Byrd P, Daughety T, Shalaby M . The mechanism of appearance of specific antibody-forming cells in lungs of inbred mice after intratracheal immunization with sheep erythrocytes. Am Rev Respir Dis. 1983; 127(3):316-21. DOI: 10.1164/arrd.1983.127.3.316. View

Furuta T, Ueda K, Kyuwa S, Fujiwara K . Effect of T-cell transfer on Pneumocystis carinii infection in nude mice. Jpn J Exp Med. 1984; 54(2):57-64. View

Bowen D, Lane H, Fauci A . Immunopathogenesis of the acquired immunodeficiency syndrome. Ann Intern Med. 1985; 103(5):704-9. DOI: 10.7326/0003-4819-103-5-704. View

Long E, Smith J, Meier J . Attachment of Pneumocystis carinii to rat pneumocytes. Lab Invest. 1986; 54(6):609-15. View

Murray J, Garay S, Hopewell P, Mills J, Snider G, Stover D . NHLBI workshop summary. Pulmonary complications of the acquired immunodeficiency syndrome: an update. Report of the second National Heart, Lung and Blood Institute workshop. Am Rev Respir Dis. 1987; 135(2):504-9. DOI: 10.1164/arrd.1987.135.2.504. View

Cushion M, Ruffolo J, Walzer P . Analysis of the developmental stages of Pneumocystis carinii, in vitro. Lab Invest. 1988; 58(3):324-31. View

10.

Roy S, Poulin L, Wainberg M . Infection of human monocytes/macrophages by HIV-1: effect on secretion of IL-1 activity. Immunology. 1988; 64(2):233-9. PMC: 1384948. View

11.

Harmsen A . Role of alveolar macrophages in lipopolysaccharide-induced neutrophil accumulation. Infect Immun. 1988; 56(8):1858-63. PMC: 259493. DOI: 10.1128/iai.56.8.1858-1863.1988. View

12.

Awwad M, NORTH R . Immunologically mediated regression of a murine lymphoma after treatment with anti-L3T4 antibody. A consequence of removing L3T4+ suppressor T cells from a host generating predominantly Lyt-2+ T cell-mediated immunity. J Exp Med. 1988; 168(6):2193-206. PMC: 2189164. DOI: 10.1084/jem.168.6.2193. View

13.

Bender B, Davidson B, Kline R, Brown C, Quinn T . Role of the mononuclear phagocyte system in the immunopathogenesis of human immunodeficiency virus infection and the acquired immunodeficiency syndrome. Rev Infect Dis. 1988; 10(6):1142-54. DOI: 10.1093/clinids/10.6.1142. View

14.

Sundberg J, BURNSTEIN T, Shultz L, Bedigian H . Identification of Pneumocystis carinii in immunodeficient mice. Lab Anim Sci. 1989; 39(3):213-8. View

15.

Masur H, Ognibene F, Yarchoan R, Shelhamer J, Baird B, Travis W . CD4 counts as predictors of opportunistic pneumonias in human immunodeficiency virus (HIV) infection. Ann Intern Med. 1989; 111(3):223-31. DOI: 10.7326/0003-4819-111-3-223. View

16.

Pintozzi R . Modified Grocott's methenamine silver nitrate method for quick staining of Pneumocystis carinii. J Clin Pathol. 1978; 31(8):803-5. PMC: 1145410. DOI: 10.1136/jcp.31.8.803. View