Escherichia Coli Dihydropyrimidine Dehydrogenase is a Novel NAD-dependent Heterotetramer Essential for the Production of 5,6-dihydrouracil

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2010 Dec 21

PMID 21169495

Citations 16

Authors

Ryota Hidese

Hisaaki Mihara

Tatsuo Kurihara

Nobuyoshi Esaki

Affiliations

Soon will be listed here.

Abstract

The reductive pyrimidine catabolic pathway is absent in Escherichia coli. However, the bacterium contains an enzyme homologous to mammalian dihydropyrimidine dehydrogenase. Here, we show that E. coli dihydropyrimidine dehydrogenase is the first member of a novel NADH-dependent subclass of iron-sulfur flavoenzymes catalyzing the conversion of uracil to 5,6-dihydrouracil in vivo.

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