Functionalization of Iron Oxide Magnetic Nanoparticles with Targeting Ligands: Their Physicochemical Properties and in Vivo Behavior

Overview

Journal Nanomedicine (Lond)

Specialty Biotechnology

Date 2010 Dec 7

PMID 21128719

Citations 25

Authors

Chen Fang

Omid Veiseh

Forrest Kievit

Narayan Bhattarai

Freddy Wang

Zach Stephen

Chun Li

Donghoon Lee

Richard G Ellenbogen

Miqin Zhang

Affiliations

Soon will be listed here.

Abstract

Aim: To develop and evaluate two tumor-specific nanoprobes by functionalization of a polyethylene glycol-immobilized nanoparticle with arginine-glycine-aspartic acid (RGD) or chlorotoxin ligand that targets α(v)β(3) integrin and matrix metalloproteinase-2 receptors, respectively.

Materials & Methods: The nanoprobes were made of iron oxide cores, biocompatible polymer coating, and surface-conjugated RGD or chlorotoxin peptide. The tumor-targeting specificity of the nanoprobes was evaluated both in vitro and in vivo.

Results & Discussion: Both nanoprobes were highly dispersive and exhibited excellent long-term stability in cell culture media. The RGD-conjugated nanoprobe displayed a strong initial accumulation near neovasculatures in tumors followed by quick clearance. Conversely, the chlorotoxin-enabled nanoprobe exhibited sustained accumulation throughout the tumor.

Conclusion: These findings revealed the influence of the targeting ligands on the intratumoral distribution of the ligand-enabled nanoprobes. With flexible surface chemistry, our nanoparticle platform can be used in a modular fashion to conjugate biomolecules for intended applications.

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