Determination of Rigidity of Protein Bound Au(144) Clusters by Electron Cryomicroscopy

Overview

Journal J Phys Chem C Nanomater Interfaces

Publisher American Chemical Society

Date 2010 Dec 1

PMID 21116473

Citations 6

Authors

Jonathan Z Sexton

Christopher J Ackerson

Affiliations

Soon will be listed here.

Abstract

A method for estimating the positional displacement of protein bound gold nanoparticles is presented and used to estimate the rigidity of linkage of Au(144) nanoparticles bound to a tetrameric model protein. We observe a distribution of displacement values where most Au(144) clusters are immobilized to within 3Å relative to the protein center of mass. The shape of the distribution suggests two physical processes of thermal motion and protein deformation. The application of this and similar rigid gold nanoparticle/protein conjugates in high resolution single particle electron cryo-microscopy is discussed.

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