Multi-parameter Fluorescence-activated Cell Sorting and Analysis of Stem and Progenitor Cells in Myeloid Malignancies

Overview

Journal Best Pract Res Clin Haematol

Publisher Elsevier

Specialty Hematology

Date 2010 Nov 30

PMID 21112038

Citations 16

Authors

Britta Will

Ulrich Steidl

Affiliations

Soon will be listed here.

Abstract

Owing to the discovery that rare leukemia-initiating cells (or leukemia stem cells, LSC) give origin to and propagate a hierarchical cellular organization of variably differentiated leukemic blasts, the analysis of precisely defined stem and progenitor cells have increasingly gained importance. Emergence of multi-parameter high-speed fluorescence-activated cell sorting (FACS) for the subfractionation of hematopoietic progenitor cells has allowed research on the biology of the cell-of-origin for LSCs and of LSCs as potential (or essential) therapeutic targets that may escape chemotherapy and consequently contribute to disease relapse. This review introduces the current state-of-the-art methods for the fractionation of hematopoietic stem and progenitor cells, with particular focus on myeloid malignancies. As many aspects of human normal and malignant hematopoiesis are frequently modeled in animal studies, we also provide an overview of hematopoietic stem and progenitor cell purification methods that are commonly utilized for research in murine models of disease.

Citing Articles

Single-cell Technology in Stem Cell Research.

Golchin A, Shams F, Moradi F, Sadrabadi A, Parviz S, Alipour S Curr Stem Cell Res Ther. 2024; 20(1):9-32.

PMID: 38243989 DOI: 10.2174/011574888X265479231127065541.

Multi-omic analyses in immune cell development with lessons learned from T cell development.

Cordes M, Pike-Overzet K, van den Akker E, Staal F, Cante-Barrett K Front Cell Dev Biol. 2023; 11:1163529.

PMID: 37091971 PMC: 10118026. DOI: 10.3389/fcell.2023.1163529.

Isolation of Murine Myeloid Progenitor Populations by CD34/CD150 Surface Markers.

Olender L, Thapa R, Gazit R Cells. 2022; 11(3).

PMID: 35159159 PMC: 8834359. DOI: 10.3390/cells11030350.

Microfluidics as efficient technology for the isolation and characterization of stem cells.

Aghlmandi A, Nikshad A, Safaralizadeh R, Warkiani M, Aghebati-Maleki L, Yousefi M EXCLI J. 2021; 20:426-443.

PMID: 33746671 PMC: 7975637. DOI: 10.17179/excli2020-3028.

Negative Regulation of the Differentiation of Flk2 CD34 LSK Hematopoietic Stem Cells by EKLF/KLF1.

Hung C, Wang K, Liou Y, Wang J, Huang A, Lee T Int J Mol Sci. 2020; 21(22).

PMID: 33182781 PMC: 7697791. DOI: 10.3390/ijms21228448.

References

Jordan C, Upchurch D, Szilvassy S, Guzman M, Howard D, PETTIGREW A . The interleukin-3 receptor alpha chain is a unique marker for human acute myelogenous leukemia stem cells. Leukemia. 2000; 14(10):1777-84. DOI: 10.1038/sj.leu.2401903. View

Spangrude G, Heimfeld S, Weissman I . Purification and characterization of mouse hematopoietic stem cells. Science. 1988; 241(4861):58-62. DOI: 10.1126/science.2898810. View

Craig W, Kay R, CUTLER R, Lansdorp P . Expression of Thy-1 on human hematopoietic progenitor cells. J Exp Med. 1993; 177(5):1331-42. PMC: 2191025. DOI: 10.1084/jem.177.5.1331. View

Berardi A, Wang A, Levine J, Lopez P, Scadden D . Functional isolation and characterization of human hematopoietic stem cells. Science. 1995; 267(5194):104-8. DOI: 10.1126/science.7528940. View

Spangrude G, Brooks D . Mouse strain variability in the expression of the hematopoietic stem cell antigen Ly-6A/E by bone marrow cells. Blood. 1993; 82(11):3327-32. View

Berenson R, Andrews R, Bensinger W, Kalamasz D, Knitter G, Buckner C . Antigen CD34+ marrow cells engraft lethally irradiated baboons. J Clin Invest. 1988; 81(3):951-5. PMC: 442551. DOI: 10.1172/JCI113409. View

Rasko J, Metcalf D, Rossner M, Begley C, Nicola N . The flt3/flk-2 ligand: receptor distribution and action on murine haemopoietic cell survival and proliferation. Leukemia. 1995; 9(12):2058-66. View

Challen G, Boles N, Lin K, Goodell M . Mouse hematopoietic stem cell identification and analysis. Cytometry A. 2008; 75(1):14-24. PMC: 2640229. DOI: 10.1002/cyto.a.20674. View

Guzman M, Neering S, Upchurch D, Grimes B, Howard D, Rizzieri D . Nuclear factor-kappaB is constitutively activated in primitive human acute myelogenous leukemia cells. Blood. 2001; 98(8):2301-7. DOI: 10.1182/blood.v98.8.2301. View

10.

Pearce D, Bonnet D . The combined use of Hoechst efflux ability and aldehyde dehydrogenase activity to identify murine and human hematopoietic stem cells. Exp Hematol. 2007; 35(9):1437-46. DOI: 10.1016/j.exphem.2007.06.002. View

11.

Jin L, Hope K, Zhai Q, Smadja-Joffe F, Dick J . Targeting of CD44 eradicates human acute myeloid leukemic stem cells. Nat Med. 2006; 12(10):1167-74. DOI: 10.1038/nm1483. View

12.

Guzman M, Rossi R, Neelakantan S, Li X, Corbett C, Hassane D . An orally bioavailable parthenolide analog selectively eradicates acute myelogenous leukemia stem and progenitor cells. Blood. 2007; 110(13):4427-35. PMC: 2234793. DOI: 10.1182/blood-2007-05-090621. View

13.

Yokota T, Oritani K, Butz S, Kokame K, Kincade P, Miyata T . The endothelial antigen ESAM marks primitive hematopoietic progenitors throughout life in mice. Blood. 2008; 113(13):2914-23. PMC: 2974489. DOI: 10.1182/blood-2008-07-167106. View

14.

Blair A, Hogge D, Sutherland H . Most acute myeloid leukemia progenitor cells with long-term proliferative ability in vitro and in vivo have the phenotype CD34(+)/CD71(-)/HLA-DR-. Blood. 1998; 92(11):4325-35. View

15.

van Rhenen A, van Dongen G, Kelder A, Rombouts E, Feller N, Moshaver B . The novel AML stem cell associated antigen CLL-1 aids in discrimination between normal and leukemic stem cells. Blood. 2007; 110(7):2659-66. DOI: 10.1182/blood-2007-03-083048. View

16.

Zhou S, Schuetz J, Bunting K, Colapietro A, Sampath J, Morris J . The ABC transporter Bcrp1/ABCG2 is expressed in a wide variety of stem cells and is a molecular determinant of the side-population phenotype. Nat Med. 2001; 7(9):1028-34. DOI: 10.1038/nm0901-1028. View

17.

Taussig D, Vargaftig J, Miraki-Moud F, Griessinger E, Sharrock K, Luke T . Leukemia-initiating cells from some acute myeloid leukemia patients with mutated nucleophosmin reside in the CD34(-) fraction. Blood. 2010; 115(10):1976-84. PMC: 2837317. DOI: 10.1182/blood-2009-02-206565. View

18.

Hess D, Meyerrose T, Wirthlin L, Craft T, Herrbrich P, Creer M . Functional characterization of highly purified human hematopoietic repopulating cells isolated according to aldehyde dehydrogenase activity. Blood. 2004; 104(6):1648-55. DOI: 10.1182/blood-2004-02-0448. View

19.

Adolfsson J, Borge O, Bryder D, Theilgaard-Monch K, Sitnicka E, Sasaki Y . Upregulation of Flt3 expression within the bone marrow Lin(-)Sca1(+)c-kit(+) stem cell compartment is accompanied by loss of self-renewal capacity. Immunity. 2001; 15(4):659-69. DOI: 10.1016/s1074-7613(01)00220-5. View

20.

Matsui W, Wang Q, Barber J, Brennan S, Smith B, Borrello I . Clonogenic multiple myeloma progenitors, stem cell properties, and drug resistance. Cancer Res. 2008; 68(1):190-7. PMC: 2603142. DOI: 10.1158/0008-5472.CAN-07-3096. View