Activation of a Beta-galactosidase Recombinant Provirus: Application to Titration of Human Immunodeficiency Virus (HIV) and HIV-infected Cells

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1990 Jun 1

PMID 2110596

Citations 45

Authors

D Rocancourt

C Bonnerot

H Jouin

M Emerman

J F Nicolas

Affiliations

Soon will be listed here.

Abstract

A quantitative bioassay for human immunodeficiency viruses has been developed on the basis of the ability of the tat gene to transactivate the expression of an integrated beta-galactosidase gene in a HeLa-CD4+ cell line. Infection by a single virion of HIV-1 or HIV-2 corresponds to a unique blue syncytium or a cell cluster detected after fixation and addition of 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (a beta-galactosidase substrate). The number of infected lymphoid cells in a culture (stimulated human peripheral blood lymphocytes and cell lines) can also be quantified by cell-to-cell transmission of HIV into the HeLa-CD4(+)-beta-galactosidase monolayer. Infections by simian immunodeficiency viruses are similarly detected. This assay has been used to determine the dose response of drugs, the half-life of HIV at 37 degrees C, and the appearance of infectious particles after virus infection.

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