Multiplexed DNA Sequence Capture of Mitochondrial Genomes Using PCR Products

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2010 Nov 25

PMID 21103372

Citations 247

Authors

Tomislav Maricic

Mark Whitten

Svante Paabo

Affiliations

Soon will be listed here.

Abstract

Background: To utilize the power of high-throughput sequencers, target enrichment methods have been developed. The majority of these require reagents and equipment that are only available from commercial vendors and are not suitable for the targets that are a few kilobases in length.

Methodology/principal Findings: We describe a novel and economical method in which custom made long-range PCR products are used to capture complete human mitochondrial genomes from complex DNA mixtures. We use the method to capture 46 complete mitochondrial genomes in parallel and we sequence them on a single lane of an Illumina GA(II) instrument.

Conclusions/significance: This method is economical and simple and particularly suitable for targets that can be amplified by PCR and do not contain highly repetitive sequences such as mtDNA. It has applications in population genetics and forensics, as well as studies of ancient DNA.

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