Defects in Nuclear Pore Assembly Lead to Activation of an Aurora B-mediated Abscission Checkpoint

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 2010 Nov 25

PMID 21098116

Citations 59

Authors

Douglas R Mackay

Masaki Makise

Katharine S Ullman

Affiliations

Soon will be listed here.

Abstract

Correct assembly of nuclear pore complexes (NPCs), which directly and indirectly control nuclear environment and architecture, is vital to genomic regulation. We previously found that nucleoporin 153 (Nup 153) is required for timely progression through late mitosis. In this study, we report that disruption of Nup 153 function by either small interfering RNA-mediated depletion or expression of a dominant-interfering Nup 153 fragment results in dramatic mistargeting of the pore basket components Tpr and Nup 50 in midbody-stage cells. We find a concomitant appearance of aberrantly localized active Aurora B and an Aurora B-dependent delay in abscission. Depletion of Nup 50 is also sufficient to increase the number of midbody-stage cells and, likewise, triggers distinctive mislocalization of Aurora B. Together, our results suggest that defects in nuclear pore assembly, and specifically the basket structure, at this time of the cell cycle activate an Aurora B-mediated abscission checkpoint, thereby ensuring that daughter cells are generated only when fully formed NPCs are present.

Citing Articles

Nuclear transport proteins: structure, function, and disease relevance.

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The Calpain-7 protease functions together with the ESCRT-III protein IST1 within the midbody to regulate the timing and completion of abscission.

Paine E, Skalicky J, Whitby F, Mackay D, Ullman K, Hill C Elife. 2023; 12.

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Mechanics and regulation of cytokinetic abscission.

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Comprehensive analysis of the human ESCRT-III-MIT domain interactome reveals new cofactors for cytokinetic abscission.

Wenzel D, Mackay D, Skalicky J, Paine E, Miller M, Ullman K Elife. 2022; 11.

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A role for Nup153 in nuclear assembly reveals differential requirements for targeting of nuclear envelope constituents.

LaJoie D, Turkmen A, Mackay D, Jensen C, Aksenova V, Niwa M Mol Biol Cell. 2022; 33(13):ar117.

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