Transmissible Gastroenteritis Virus (TGEV)-based Vectors with Engineered Murine Tropism Express the Rotavirus VP7 Protein and Immunize Mice Against Rotavirus

Overview

Journal Virology

Specialty Microbiology

Date 2010 Nov 25

PMID 21094967

Citations 8

Authors

Juan Manuel Ribes

Javier Ortego

Juan Ceriani

Rebeca Montava

Luis Enjuanes

Javier Buesa

Affiliations

Soon will be listed here.

Abstract

A coronavirus vector based on the genome of the porcine transmissible gastroenteritis virus (TGEV) expressing the rotavirus VP7 protein was constructed to immunize and protect against rotavirus infections in a murine model. The tropism of this TGEV-derived vector was modified by replacing the spike S protein with the homologous protein from mouse hepatitis virus (MHV). The rotavirus gene encoding the VP7 protein was cloned into the coronavirus cDNA. BALB/c and STAT1-deficient mice were inoculated with the recombinant viral vector rTGEV(S-MHV)-VP7, which replicates in the intestine and spreads to other organs such as liver, spleen and lungs. TGEV-specific antibodies were detected in all the inoculated BALB/c mice, while rotavirus-specific antibodies were found only after immunization by the intraperitoneal route. Partial protection against rotavirus-induced diarrhea was achieved in suckling BALB/c mice born to dams immunized with the recombinant virus expressing VP7 when they were orally challenged with the homotypic rotavirus strain.

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