Recombinant Avian Infectious Bronchitis Virus Expressing a Heterologous Spike Gene Demonstrates That the Spike Protein is a Determinant of Cell Tropism

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2003 Jul 30

PMID 12885925

Citations 153

Authors

Rosa Casais

Brian Dove

David Cavanagh

Paul Britton

Affiliations

Soon will be listed here.

Abstract

A recombinant infectious bronchitis virus (IBV), BeauR-M41(S), was generated using our reverse genetics system (R. Casais, V. Thiel, S. G. Siddell, D. Cavanagh, and P. Britton, J. Virol. 75:12359-12369, 2001), in which the ectodomain region of the spike gene from IBV M41-CK replaced the corresponding region of the IBV Beaudette genome. BeauR-M41(S) acquired the same cell tropism phenotype as IBV M41-CK in four different cell types, demonstrating that the IBV spike glycoprotein is a determinant of cell tropism.

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