Dictyostelium Dynamin B Modulates Cytoskeletal Structures and Membranous Organelles

Overview

Journal Cell Mol Life Sci

Publisher Springer

Specialty Biology

Date 2010 Nov 19

PMID 21086149

Citations 12

Authors

Amrita Rai

Heike Nothe

Nikolay Tzvetkov

Elena Korenbaum

Dietmar J Manstein

Affiliations

Soon will be listed here.

Abstract

Dictyostelium discoideum cells produce five dynamin family proteins. Here, we show that dynamin B is the only member of this group of proteins that is initially produced as a preprotein and requires processing by mitochondrial proteases for formation of the mature protein. Our results show that dynamin B-depletion affects many aspects of cell motility, cell-cell and cell-surface adhesion, resistance to osmotic shock, and fatty acid metabolism. The mature form of dynamin B mediates a wide range and unique combination of functions. Dynamin B affects events at the plasma membrane, peroxisomes, the contractile vacuole system, components of the actin-based cytoskeleton, and cell adhesion sites. The modulating effect of dynamin B on the activity of the contractile vacuole system is unique for the Dictyostelium system. Other functions displayed by dynamin B are commonly associated with either classical dynamins or dynamin-related proteins.

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