Genome Sequence of Kitasatospora Setae NBRC 14216T: an Evolutionary Snapshot of the Family Streptomycetaceae

Overview

Journal DNA Res

Publisher Oxford University Press

Specialties Genetics
Molecular Biology

Date 2010 Nov 10

PMID 21059706

Citations 27

Authors

Natsuko Ichikawa

Akio Oguchi

Haruo Ikeda

Jun Ishikawa

Shigeru Kitani

Yumi Watanabe

Sanae Nakamura

Yoko Katano

Emi Kishi

Machi Sasagawa

Akiho Ankai

Shigehiro Fukui

Yoshimi Hashimoto

Sachi Kamata

Misa Otoguro

Satoshi Tanikawa

Takuya Nihira

Sueharu Horinouchi

Yasuo Ohnishi

Masayuki Hayakawa

Tomohisa Kuzuyama

Akira Arisawa

Fumiki Nomoto

Hiromi Miura

Yoko Takahashi

Nobuyuki Fujita

Affiliations

Soon will be listed here.

Abstract

Kitasatospora setae NBRC 14216(T) (=KM-6054(T)) is known to produce setamycin (bafilomycin B1) possessing antitrichomonal activity. The genus Kitasatospora is morphologically similar to the genus Streptomyces, although they are distinguishable from each other on the basis of cell wall composition and the 16S rDNA sequence. We have determined the complete genome sequence of K. setae NBRC 14216(T) as the first Streptomycetaceae genome other than Streptomyces. The genome is a single linear chromosome of 8,783,278 bp with terminal inverted repeats of 127,148 bp, predicted to encode 7569 protein-coding genes, 9 rRNA operons, 1 tmRNA and 74 tRNA genes. Although these features resemble those of Streptomyces, genome-wide comparison of orthologous genes between K. setae and Streptomyces revealed smaller extent of synteny. Multilocus phylogenetic analysis based on amino acid sequences unequivocally placed K. setae outside the Streptomyces genus. Although many of the genes related to morphological differentiation identified in Streptomyces were highly conserved in K. setae, there were some differences such as the apparent absence of the AmfS (SapB) class of surfactant protein and differences in the copy number and variation of paralogous components involved in cell wall synthesis.

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