The Adh Gene Promoters of Drosophila Melanogaster and Drosophila Orena Are Functionally Conserved and Share Features of Sequence Structure and Nuclease-protected Sites

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1990 Feb 1

PMID 2105454

Citations 14

Authors

K Moses

U Heberlein

M Ashburner

Affiliations

Soon will be listed here.

Abstract

The sibling species Drosophila melanogaster and D. orena show similar patterns of alcohol dehydrogenase expression, both spatially and temporally. These two species diverged from a common ancestor 6 million to 15 million years ago, and the DNA sequences of the promoter regions of their Adh genes show a mosaic pattern of conservation and change. By interspecific transformation of D. orena sequences into D. melanogaster, we demonstrate a functional equivalence between these sequences. Using both D. melanogaster embryo extracts and purified transcription factor Adf-1, we compare the protection of these promoter sequences from nuclease, demonstrating considerable conservation.

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