A Ferredoxin-type Iron-sulfur Protein Gene, Frx B, is Expressed in the Chloroplasts of Tobacco and Spinach

Overview

Journal Plant Mol Biol

Date 1990 Sep 1

PMID 2103463

Citations 2

Authors

C H Lin

M Wu

Affiliations

Soon will be listed here.

Abstract

Previously, a ferredoxin-type iron-sulfur protein, frx B protein, was identified in a high-salt extract of the purified thylakoid membrane of Chlamydomonas reinhardtii, a unicellular green alga. Polyclonal antibody was raised against a synthetic pentadecameric peptide with an amino acid sequence corresponding to the highly conserved region of the putative frx B proteins of 3 land plants. In this report, protein(s) reacting strongly and specifically with this antibody was detected in the equivalent high-salt extract prepared from purified chloroplast of spinach and tobacco. One strong reaction polypeptide band from tobacco chloroplast was purified from SDS-polyacrylamide gel and subjected to endoproteinase lys C digestion. The resulting polypeptides were separated by reversed-phase chromatography. N-terminal sequencing of 3 purified polypeptides revealed that the protein is encoded by the 'frxB gene' identified from DNA sequence analysis.

Citing Articles

Loss of all ndh genes as determined by sequencing the entire chloroplast genome of the black pine Pinus thunbergii.

Wakasugi T, Tsudzuki J, Ito S, Nakashima K, Tsudzuki T, Sugiura M Proc Natl Acad Sci U S A. 1994; 91(21):9794-8.

PMID: 7937893 PMC: 44903. DOI: 10.1073/pnas.91.21.9794.

Internal editing of the maize chloroplast ndhA transcript restores codons for conserved amino acids.

Maier R, Hoch B, Zeltz P, Kossel H Plant Cell. 1992; 4(5):609-16.

PMID: 1498612 PMC: 160157. DOI: 10.1105/tpc.4.5.609.

References

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Nie Z, Chang D, Wu M . Protein-DNA interaction within one cloned chloroplast DNA replication origin of Chlamydomonas. Mol Gen Genet. 1987; 209(2):265-9. DOI: 10.1007/BF00329652. View

Chomyn A, Mariottini P, Cleeter M, Ragan C, HATEFI Y, Doolittle R . Six unidentified reading frames of human mitochondrial DNA encode components of the respiratory-chain NADH dehydrogenase. Nature. 1985; 314(6012):592-7. DOI: 10.1038/314592a0. View

Dunn P, Gray J . Nucleotide sequence of the frxB gene in wheat chloroplast DNA. Nucleic Acids Res. 1988; 16(1):348. PMC: 334632. DOI: 10.1093/nar/16.1.348. View

Aebersold R, Teplow D, HOOD L, Kent S . Electroblotting onto activated glass. High efficiency preparation of proteins from analytical sodium dodecyl sulfate-polyacrylamide gels for direct sequence analysis. J Biol Chem. 1986; 261(9):4229-38. View

Hager D, Burgess R . Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other.... Anal Biochem. 1980; 109(1):76-86. DOI: 10.1016/0003-2697(80)90013-5. View

Wu M, Nie Z, Yang J . The 18-kD protein that binds to the chloroplast DNA replicative origin is an iron-sulfur protein related to a subunit of NADH dehydrogenase. Plant Cell. 1989; 1(5):551-7. PMC: 159789. DOI: 10.1105/tpc.1.5.551. View

Towbin H, Staehelin T, Gordon J . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9):4350-4. PMC: 411572. DOI: 10.1073/pnas.76.9.4350. View

Shinozaki K, Ohme M, Tanaka M, Wakasugi T, Hayashida N, Matsubayashi T . The complete nucleotide sequence of the tobacco chloroplast genome: its gene organization and expression. EMBO J. 1986; 5(9):2043-2049. PMC: 1167080. DOI: 10.1002/j.1460-2075.1986.tb04464.x. View

10.

Gfeller R, Gibbs M . Fermentative Metabolism of Chlamydomonas reinhardtii: II. Role of Plastoquinone. Plant Physiol. 1985; 77(2):509-11. PMC: 1064549. DOI: 10.1104/pp.77.2.509. View

11.

Hsieh J, Lin F, Tam M . Electroblotting onto glass-fiber filter from an analytical isoelectrofocusing gel: a preparative method for isolating proteins for N-terminal microsequencing. Anal Biochem. 1988; 170(1):1-8. DOI: 10.1016/0003-2697(88)90082-6. View

12.

Matsubayashi T, Wakasugi T, Shinozaki K, Yamaguchi-Shinozaki K, Zaita N, Hidaka T . Six chloroplast genes (ndhA-F) homologous to human mitochondrial genes encoding components of the respiratory chain NADH dehydrogenase are actively expressed: determination of the splice sites in ndhA and ndhB pre-mRNAs. Mol Gen Genet. 1987; 210(3):385-93. DOI: 10.1007/BF00327187. View

13.

Bennoun P . Evidence for a respiratory chain in the chloroplast. Proc Natl Acad Sci U S A. 1982; 79(14):4352-6. PMC: 346669. DOI: 10.1073/pnas.79.14.4352. View

14.

Garab G, Lajko F, Mustardy L, Marton L . Respiratory control over photosynthetic electron transport in chloroplasts of higher-plant cells: evidence for chlororespiration. Planta. 2013; 179(3):349-58. DOI: 10.1007/BF00391080. View

15.

Chua N, Bennoun P . Thylakoid membrane polypeptides of Chlamydomonas reinhardtii: wild-type and mutant strains deficient in photosystem II reaction center. Proc Natl Acad Sci U S A. 1975; 72(6):2175-9. PMC: 432719. DOI: 10.1073/pnas.72.6.2175. View

16.

Maione T, Gibbs M . Association of the Chloroplastic Respiratory and Photosynthetic Electron Transport Chains of Chlamydomonas reinhardii with Photoreduction and the Oxyhydrogen Reaction. Plant Physiol. 1986; 80(2):364-8. PMC: 1075118. DOI: 10.1104/pp.80.2.364. View

17.

Lemaire C, Wollman F, Bennoun P . Restoration of phototrophic growth in a mutant of Chlamydomonas reinhardtii in which the chloroplast atpB gene of the ATP synthase has a deletion: an example of mitochondria-dependent photosynthesis. Proc Natl Acad Sci U S A. 1988; 85(5):1344-8. PMC: 279767. DOI: 10.1073/pnas.85.5.1344. View

18.

Thompson J . Isolation and characterization of DNA from different plastid types of Tropaecolum majus. Eur J Cell Biol. 1980; 21(1):37-42. View