Internal Editing of the Maize Chloroplast NdhA Transcript Restores Codons for Conserved Amino Acids

Overview

Journal Plant Cell

Publisher Oxford University Press

Specialties Biology
Cell Biology

Date 1992 May 1

PMID 1498612

Citations 41

Authors

R M Maier

B Hoch

P Zeltz

H Kossel

Affiliations

Soon will be listed here.

Abstract

The NADH dehydrogenase subunit A (ndhA) gene from maize chloroplasts encodes a highly conserved peptide, which at several positions could be restored to consensus sequences by potential C-to-U editing of the codons involved. This gene was, therefore, chosen for analysis of its mRNA sequence in the form of amplified cDNA. A comparison of this cDNA sequence with the plastome-encoded ndhA sequence reveals four C-to-U editing sites, thereby demonstrating as a novel finding that chloroplast editing can also affect internal mRNA positions. All the edited codons restore amino acids that are conserved in the ndhA-encoded peptides of other chloroplast species. Alignment with homologous mitochondrial NADH-ubiquinone reductase subunit 1 (nad1) sequences of plant and even nonplant species shows that two of the editing positions restore universally conserved amino acids and that one editing site is even shared with nad1 mRNA of plant mitochondria. No editing sites could be detected in the cDNA derived from transcripts of the maize chloroplast RNA polymerase alpha-subunit (rpoA) gene.

Citing Articles

Analysis of the RNA Editing Sites and Orthologous Gene Function of Transcriptome and Chloroplast Genomes in the Evolution of Five Species.

Cai H, Ren Y, Du J, Liu L, Long L, Yang M Int J Mol Sci. 2023; 24(16).

PMID: 37629135 PMC: 10454583. DOI: 10.3390/ijms241612954.

Identification and analysis of RNA editing sites in chloroplast transcripts of kenaf ( L.).

Tang D, Wei F, Haneef Kashif M, Munsif F, Zhou R 3 Biotech. 2019; 9(10):361.

PMID: 31559110 PMC: 6742680. DOI: 10.1007/s13205-019-1893-3.

Identification and Analysis of RNA Editing Sites in the Chloroplast Transcripts of Aegilops tauschii L.

Wang M, Liu H, Ge L, Xing G, Wang M, Weining S Genes (Basel). 2017; 8(1).

PMID: 28042823 PMC: 5295008. DOI: 10.3390/genes8010013.

Abundant RNA editing sites of chloroplast protein-coding genes in Ginkgo biloba and an evolutionary pattern analysis.

He P, Huang S, Xiao G, Zhang Y, Yu J BMC Plant Biol. 2016; 16(1):257.

PMID: 27903241 PMC: 5131507. DOI: 10.1186/s12870-016-0944-8.

Differential regulation of Arabidopsis plastid gene expression and RNA editing in non-photosynthetic tissues.

Tseng C, Lee C, Chung Y, Sung T, Hsieh M Plant Mol Biol. 2013; 82(4-5):375-92.

PMID: 23645360 DOI: 10.1007/s11103-013-0069-5.

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