The Maize Zein Gene ZE19 Contains Two Distinct Promoters Which Are Independently Activated in Endosperm and Anthers of Transgenic Petunia Plants

Overview

Journal Plant Mol Biol

Date 1990 Jul 1

PMID 2103445

Citations 16

Authors

F. Quattrocchio

M A Tolk

I Coraggio

J N Mol

A Viotti

R E Koes

Affiliations

Soon will be listed here.

Abstract

The activity, tissue specificity and temporal expression of the tandem promoter region preceding a maize zein gene (zE19, encoding a 19 kDa zein protein) were tested in transgenic Petunia plants. To simplify the analysis, the tandem promoter as well as each of the two separate promoter regions were fused to the beta-glucuronidase (GUS) reporter gene. All of the three constructs directed the synthesis of GUS in the endosperm of transformed seeds indicating that both separate promoters are independently activated and show the same tissue and cell type specificity observed for zein genes in maize. The kinetics of accumulation and the localization of GUS activity are not coordinated with those of Petunia endogenous seed storage proteins during the development of transformed seeds. Unexpectedly, we detected high levels of GUS activity in anthers of transformed Petunia plants for all three constructs. This appears to reflect the expression pattern of zein genes in maize, since we detect zein transcripts in anthers. Finally, we discuss the possible origin and function of the tandem promoter arrangement on the basis of these data.

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References

Jones R . Effects of floury-2 locus on zein accumulation and RNA metabolism during maize endosperm development. Biochem Genet. 1978; 16(1-2):27-38. DOI: 10.1007/BF00484382. View

van Tunen A, Koes R, Spelt C, van der Krol A, Stuitje A, Mol J . Cloning of the two chalcone flavanone isomerase genes from Petunia hybrida: coordinate, light-regulated and differential expression of flavonoid genes. EMBO J. 1988; 7(5):1257-63. PMC: 458371. DOI: 10.1002/j.1460-2075.1988.tb02939.x. View

Koes R, Spelt C, Mol J . The chalcone synthase multigene family of Petunia hybrida (V30): differential, light-regulated expression during flower development and UV light induction. Plant Mol Biol. 2013; 12(2):213-25. DOI: 10.1007/BF00020506. View

Schernthaner J, Matzke M, Matzke A . Endosperm-specific activity of a zein gene promoter in transgenic tobacco plants. EMBO J. 1988; 7(5):1249-55. PMC: 458370. DOI: 10.1002/j.1460-2075.1988.tb02938.x. View

Marks M, Lindell J, Larkins B . Quantitative analysis of the accumulation of Zein mRNA during maize endosperm development. J Biol Chem. 1985; 260(30):16445-50. View

Hagen G, Rubenstein I . Complex organization of zein genes in maize. Gene. 1981; 13(3):239-49. DOI: 10.1016/0378-1119(81)90029-9. View

van Tunen A, Hartman S, Mur L, Mol J . Regulation of chalcone flavanone isomerase (CHI) gene expression inPetunia hybrida: the use of alternative promoters in corolla, anthers and pollen. Plant Mol Biol. 2013; 12(5):539-51. DOI: 10.1007/BF00036968. View

Coraggio I, Compagno C, Martegani E, Ranzi B, Sala E, Alberghina L . Transcription and expression of zein sequences in yeast under natural plant or yeast promoters. EMBO J. 1986; 5(3):459-65. PMC: 1166786. DOI: 10.1002/j.1460-2075.1986.tb04234.x. View

Viotti A, Abildsten D, Pogna N, Sala E, Pirrotta V . Multiplicity and diversity of cloned zein cDNA sequences and their chromosomal localisation. EMBO J. 1982; 1(1):53-8. PMC: 552994. DOI: 10.1002/j.1460-2075.1982.tb01123.x. View

10.

Langridge P, Eibel H, Brown J, Feix G . Transcription from maize storage protein gene promoters in yeast. EMBO J. 1984; 3(11):2467-71. PMC: 557713. DOI: 10.1002/j.1460-2075.1984.tb02157.x. View

11.

Viotti A, Sala E, Marotta R, Alberi P, Balducci C, Soave C . Genes and mRNAs coding for zein polypeptides in Zea mays. Eur J Biochem. 1979; 102(1):211-22. DOI: 10.1111/j.1432-1033.1979.tb06282.x. View

12.

Maier U, Brown J, Tologcyzki C, Feix G . Binding of a nuclear factor to a consensus sequence in the 5' flanking region of zein genes from maize. EMBO J. 1987; 6(1):17-22. PMC: 553350. DOI: 10.1002/j.1460-2075.1987.tb04712.x. View

13.

Schmidt R, Burr F, Aukerman M, Burr B . Maize regulatory gene opaque-2 encodes a protein with a "leucine-zipper" motif that binds to zein DNA. Proc Natl Acad Sci U S A. 1990; 87(1):46-50. PMC: 53196. DOI: 10.1073/pnas.87.1.46. View

14.

Tsai C, Larkins B, Glover D . Interaction of the opaque-2 gene with starch-forming mutant genes on the synthesis of zein in maize endosperm. Biochem Genet. 1978; 16(9-10):883-96. DOI: 10.1007/BF00483740. View

15.

Jones R, Larkins B, Tsai C . Storage Protein Synthesis in Maize: II. Reduced Synthesis of a Major Zein Component by the Opaque-2 Mutant of Maize. Plant Physiol. 1977; 59(4):525-9. PMC: 542441. DOI: 10.1104/pp.59.4.525. View

16.

Bevan M . Binary Agrobacterium vectors for plant transformation. Nucleic Acids Res. 1984; 12(22):8711-21. PMC: 320409. DOI: 10.1093/nar/12.22.8711. View

17.

Jefferson R, Kavanagh T, Bevan M . GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 1987; 6(13):3901-7. PMC: 553867. DOI: 10.1002/j.1460-2075.1987.tb02730.x. View

18.

Spena A, Viotti A, Pirrotta V . Two adjacent genomic zein sequences: structure, organization and tissue-specific restriction pattern. J Mol Biol. 1983; 169(4):799-811. DOI: 10.1016/s0022-2836(83)80137-5. View

19.

Beachy R, Chen Z, Horsch R, Rogers S, Hoffmann N, Fraley R . Accumulation and assembly of soybean beta-conglycinin in seeds of transformed petunia plants. EMBO J. 1985; 4(12):3047-53. PMC: 554621. DOI: 10.1002/j.1460-2075.1985.tb04044.x. View

20.

Spena A, Viotti A, Pirrotta V . A homologous repetitive block structure underlies the heterogeneity of heavy and light chain zein genes. EMBO J. 1982; 1(12):1589-94. PMC: 553256. DOI: 10.1002/j.1460-2075.1982.tb01360.x. View