DNA Methylation in the Alcohol Dehydrogenase-1 Gene of Maize

Overview

Journal Plant Mol Biol

Date 1990 Jul 1

PMID 2103426

Citations 5

Authors

V Walbot

C Warren

Affiliations

Soon will be listed here.

Abstract

Using a battery of methylation-sensitive restriction enzymes, cytosine methylation at 23 sites in a 7.6 kb region surrounding the Alcohol dehydrogenase-1 (Adh1) gene was measured in DNA prepared from immature maize cobs. Both the 5' upstream region and the entire coding region were hypomethylated in the two alleles examined. Methylation in Adh1 is independent of changes in Mutator transposable element methylation. The role of DNA methylation in Adh1 gene regulation is discussed.

Citing Articles

Characteristics of the tomato nuclear genome as determined by sequencing undermethylated EcoRI digested fragments.

Wang Y, van der Hoeven R, Nielsen R, Mueller L, Tanksley S Theor Appl Genet. 2005; 112(1):72-84.

PMID: 16208505 DOI: 10.1007/s00122-005-0107-z.

Differential methylation of genes and repeats in land plants.

Rabinowicz P, Citek R, Budiman M, Nunberg A, Bedell J, Lakey N Genome Res. 2005; 15(10):1431-40.

PMID: 16204196 PMC: 1240086. DOI: 10.1101/gr.4100405.

Genes and transposons are differentially methylated in plants, but not in mammals.

Rabinowicz P, Palmer L, May B, Hemann M, Lowe S, McCombie W Genome Res. 2003; 13(12):2658-64.

PMID: 14656970 PMC: 403807. DOI: 10.1101/gr.1784803.

Cresse A, Hulbert S, Brown W, Lucas J, Bennetzen J Genetics. 1995; 140(1):315-24.

PMID: 7635296 PMC: 1206558. DOI: 10.1093/genetics/140.1.315.

Reactivation of Mutator transposable elements of maize by ultraviolet light.

Walbot V Mol Gen Genet. 1992; 234(3):353-60.

PMID: 1328840 DOI: 10.1007/BF00538694.

References

Otto S, Walbot V . DNA methylation in eukaryotes: kinetics of demethylation and de novo methylation during the life cycle. Genetics. 1990; 124(2):429-37. PMC: 1203935. DOI: 10.1093/genetics/124.2.429. View

CHANDLER V, Walbot V . DNA modification of a maize transposable element correlates with loss of activity. Proc Natl Acad Sci U S A. 1986; 83(6):1767-71. PMC: 323165. DOI: 10.1073/pnas.83.6.1767. View

Walker J, Howard E, Dennis E, Peacock W . DNA sequences required for anaerobic expression of the maize alcohol dehydrogenase 1 gene. Proc Natl Acad Sci U S A. 1987; 84(19):6624-8. PMC: 299135. DOI: 10.1073/pnas.84.19.6624. View

Walbot V . Inheritance of mutator activity in Zea mays as assayed by somatic instability of the bz2-mu1 allele. Genetics. 1986; 114(4):1293-312. PMC: 1203041. DOI: 10.1093/genetics/114.4.1293. View

Walbot V, Warren C . Regulation of Mu element copy number in maize lines with an active or inactive Mutator transposable element system. Mol Gen Genet. 1988; 211(1):27-34. DOI: 10.1007/BF00338389. View

Nelson M, McClelland M . Effect of site-specific methylation on DNA modification methyltransferases and restriction endonucleases. Nucleic Acids Res. 1989; 17 Suppl:r389-415. PMC: 334788. DOI: 10.1093/nar/17.suppl.r389. View

Holler M, Westin G, Jiricny J, Schaffner W . Sp1 transcription factor binds DNA and activates transcription even when the binding site is CpG methylated. Genes Dev. 1988; 2(9):1127-35. DOI: 10.1101/gad.2.9.1127. View

Ferl R, Nick H . In vivo detection of regulatory factor binding sites in the 5' flanking region of maize Adh1. J Biol Chem. 1987; 262(17):7947-50. View

Chomet P, Wessler S, Dellaporta S . Inactivation of the maize transposable element Activator (Ac) is associated with its DNA modification. EMBO J. 1987; 6(2):295-302. PMC: 553394. DOI: 10.1002/j.1460-2075.1987.tb04753.x. View

10.

Roberts J, Callis J, Wemmer D, Walbot V, Jardetzky O . Mechanisms of cytoplasmic pH regulation in hypoxic maize root tips and its role in survival under hypoxia. Proc Natl Acad Sci U S A. 1984; 81(11):3379-83. PMC: 345511. DOI: 10.1073/pnas.81.11.3379. View

11.

Cone K, Burr F, Burr B . Molecular analysis of the maize anthocyanin regulatory locus C1. Proc Natl Acad Sci U S A. 1986; 83(24):9631-5. PMC: 387194. DOI: 10.1073/pnas.83.24.9631. View

12.

Freeling M, Bennett D . Maize Adh1. Annu Rev Genet. 1985; 19:297-323. DOI: 10.1146/annurev.ge.19.120185.001501. View

13.

Sachs M, Dennis E, Gerlach W, Peacock W . Two Alleles of Maize ALCOHOL DEHYDROGENASE 1 Have 3' Structural and Poly(a) Addition Polymorphisms. Genetics. 1986; 113(2):449-67. PMC: 1202849. DOI: 10.1093/genetics/113.2.449. View

14.

Boudraa M, Perrin P . CpG and TpA frequencies in the plant system. Nucleic Acids Res. 1987; 15(14):5729-37. PMC: 306018. DOI: 10.1093/nar/15.14.5729. View

15.

Dynan W . Understanding the molecular mechanism by which methylation influences gene expression. Trends Genet. 1989; 5(2):35-6. DOI: 10.1016/0168-9525(89)90016-4. View

16.

Dennis E, Gerlach W, Pryor A, Bennetzen J, Inglis A, Llewellyn D . Molecular analysis of the alcohol dehydrogenase (Adh1) gene of maize. Nucleic Acids Res. 1984; 12(9):3983-4000. PMC: 318805. DOI: 10.1093/nar/12.9.3983. View

17.

BANKS J, Masson P, Fedoroff N . Molecular mechanisms in the developmental regulation of the maize Suppressor-mutator transposable element. Genes Dev. 1988; 2(11):1364-80. DOI: 10.1101/gad.2.11.1364. View

18.

Antequera F, Bird A . Unmethylated CpG islands associated with genes in higher plant DNA. EMBO J. 1988; 7(8):2295-9. PMC: 457093. DOI: 10.1002/j.1460-2075.1988.tb03072.x. View