Mu1-related Transposable Elements of Maize Preferentially Insert into Low Copy Number DNA

Overview

Journal Genetics

Publisher Oxford University Press

Specialty Genetics

Date 1995 May 1

PMID 7635296

Citations 40

Authors

A D Cresse

S H Hulbert

W E Brown

J R Lucas

J L Bennetzen

Affiliations

Soon will be listed here.

Abstract

The Mutator transposable element system of maize was originally identified through its induction of mutations at an exceptionally high frequency and at a wide variety of loci. The Mu1 subfamily of transposable elements within this system are responsible for the majority of Mutator-induced mutations. Mu 1-related elements were isolated from active Mutator plants and their flanking DNA was characterized. Sequence analyses revealed perfect nine base target duplications directly flanking the insert for 13 of the 14 elements studied. Hybridizational studies indicated that Mu1-like elements insert primarily into regions of the maize genome that are of low copy number. This preferential selection of low copy number DNA as targets for Mu element insertion was not directed by any specific secondary structure(s) that could be detected in this study, but the 9-bp target duplications exhibited a discernibly higher than random match with the consensus sequence 5'-G-T-T-G-G/C-A-G-G/A-G-3'.

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References

Alleman M, Freeling M . The Mu transposable elements of maize: evidence for transposition and copy number regulation during development. Genetics. 1986; 112(1):107-19. PMC: 1202684. DOI: 10.1093/genetics/112.1.107. View

Nacken W, Piotrowiak R, Saedler H, Sommer H . The transposable element Tam1 from Antirrhinum majus shows structural homology to the maize transposon En/Spm and has no sequence specificity of insertion. Mol Gen Genet. 1991; 228(1-2):201-8. DOI: 10.1007/BF00282466. View

Cone K, Schmidt R, Burr B, Burr F . Advantages and limitations of using Spm as a transposon tag. Basic Life Sci. 1988; 47:149-59. DOI: 10.1007/978-1-4684-5550-2_11. View

Covarrubias L, Bolivar F . Construction and characterization of new cloning vehicles. VI. Plasmid pBR329, a new derivative of pBR328 lacking the 482-base-pair inverted duplication. Gene. 1982; 17(1):79-89. DOI: 10.1016/0378-1119(82)90103-2. View

Lee L, Fenoll C, Bennetzen J . Construction and Homologous Expression of a Maize Adh1 Based NcoI Cassette Vector. Plant Physiol. 1987; 85(2):327-30. PMC: 1054254. DOI: 10.1104/pp.85.2.327. View

Walbot V . The Mutator transposable element family of maize. Genet Eng (N Y). 1991; 13:1-37. DOI: 10.1007/978-1-4615-3760-1_1. View

Doseff A, Martienssen R, Sundaresan V . Somatic excision of the Mu1 transposable element of maize. Nucleic Acids Res. 1991; 19(3):579-84. PMC: 333651. DOI: 10.1093/nar/19.3.579. View

Chomet P, Lisch D, Hardeman K, CHANDLER V, Freeling M . Identification of a regulatory transposon that controls the Mutator transposable element system in maize. Genetics. 1991; 129(1):261-70. PMC: 1204575. DOI: 10.1093/genetics/129.1.261. View

Chen C, Oishi K, Kloeckener-Gruissem B, Freeling M . Organ-specific expression of maize Adh1 is altered after a Mu transposon insertion. Genetics. 1987; 116(3):469-77. PMC: 1203158. DOI: 10.1093/genetics/116.3.469. View

10.

Greenblatt I, Brink R . Twin Mutations in Medium Variegated Pericarp Maize. Genetics. 1962; 47(4):489-501. PMC: 1210347. DOI: 10.1093/genetics/47.4.489. View

11.

Jin Y, Bennetzen J . Integration and nonrandom mutation of a plasma membrane proton ATPase gene fragment within the Bs1 retroelement of maize. Plant Cell. 1994; 6(8):1177-86. PMC: 160511. DOI: 10.1105/tpc.6.8.1177. View

12.

Hardeman K, CHANDLER V . Two maize genes are each targeted predominantly by distinct classes of Mu elements. Genetics. 1993; 135(4):1141-50. PMC: 1205745. DOI: 10.1093/genetics/135.4.1141. View

13.

Nash J, Luehrsen K, Walbot V . Bronze-2 gene of maize: reconstruction of a wild-type allele and analysis of transcription and splicing. Plant Cell. 1990; 2(11):1039-49. PMC: 159952. DOI: 10.1105/tpc.2.11.1039. View

14.

Talbert L, Patterson G, CHANDLER V . Mu transposable elements are structurally diverse and distributed throughout the genus Zea. J Mol Evol. 1989; 29(1):28-39. DOI: 10.1007/BF02106179. View

15.

Bennetzen J, Schrick K, Springer P, Brown W, SanMiguel P . Active maize genes are unmodified and flanked by diverse classes of modified, highly repetitive DNA. Genome. 1994; 37(4):565-76. DOI: 10.1139/g94-081. View

16.

Van Schaik N, Brink R . Transpositions of Modulator, a Component of the Variegated Pericarp Allele in Maize. Genetics. 1959; 44(4):725-38. PMC: 1209976. DOI: 10.1093/genetics/44.4.725. View

17.

Doring H, Starlinger P . Barbara McClintock's controlling elements: now at the DNA level. Cell. 1984; 39(2 Pt 1):253-9. DOI: 10.1016/0092-8674(84)90002-3. View

18.

Holmes D, Quigley M . A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem. 1981; 114(1):193-7. DOI: 10.1016/0003-2697(81)90473-5. View

19.

Feinberg A, Vogelstein B . A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983; 132(1):6-13. DOI: 10.1016/0003-2697(83)90418-9. View

20.

Bennetzen J, Swanson J, Taylor W, Freeling M . DNA insertion in the first intron of maize Adh1 affects message levels: cloning of progenitor and mutant Adh1 alleles. Proc Natl Acad Sci U S A. 1984; 81(13):4125-8. PMC: 345381. DOI: 10.1073/pnas.81.13.4125. View