Suppression of Ongoing Experimental Arthritis by a Chinese Herbal Formula (huo-luo-xiao-ling Dan) Involves Changes in Antigen-induced Immunological and Biochemical Mediators of Inflammation

Overview

Journal Evid Based Complement Alternat Med

Specialty Rehabilitation Medicine

Date 2010 Oct 29

PMID 20981317

Citations 19

Authors

Ying-Hua Yang

Rajesh Rajaiah

David Y-W Lee

Zhongze Ma

Hua Yu

Harry H S Fong

Lixing Lao

Brian M Berman

Kamal D Moudgil

Affiliations

Soon will be listed here.

Abstract

Rheumatoid arthritis (RA) is one of the major autoimmune diseases of global prevalence. The use of the anti-inflammatory drugs for the treatment of RA is associated with severe adverse reactions and toxicity. This limitation has necessitated the search for novel therapeutic products. We report here a traditional Chinese medicine-based herbal formula, Huo luo xiao ling dan (HLXL), which has potent antiarthritic activity as validated in the rat adjuvant-induced arthritis (AA) model. HLXL (2.3 g/Kg) was fed to Lewis (RT.1(1)) rats daily by gavage beginning at the onset of arthritis and then continued through the observation period. HLXL inhibited the severity of ongoing AA. This suppression of arthritis was associated with significant alterations in the T cell proliferative and cytokine responses as well as the antibody response against the disease-related antigen, mycobacterial heat-shock protein 65 (Bhsp65). There was a reduction in the level of the proinflammatory cytokines IL-17 and IL-1β but enhancement of the anti-inflammatory cytokine IL-10 level. In addition, there was inhibition of both the anti-Bhsp65 antibody response and the serum level of nitric oxide. Thus, HLXL is a promising CAM modality for further testing in RA patients.

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References

Park H, Li Z, Yang X, Chang S, Nurieva R, Wang Y . A distinct lineage of CD4 T cells regulates tissue inflammation by producing interleukin 17. Nat Immunol. 2005; 6(11):1133-41. PMC: 1618871. DOI: 10.1038/ni1261. View

Brahn E, Banquerigo M, Firestein G, Boyle D, Salzman A, Szabo C . Collagen induced arthritis: reversal by mercaptoethylguanidine, a novel antiinflammatory agent with a combined mechanism of action. J Rheumatol. 1998; 25(9):1785-93. View

Olsen N, Stein C . New drugs for rheumatoid arthritis. N Engl J Med. 2004; 350(21):2167-79. DOI: 10.1056/NEJMra032906. View

OShea J, Ma A, Lipsky P . Cytokines and autoimmunity. Nat Rev Immunol. 2002; 2(1):37-45. DOI: 10.1038/nri702. View

Koenders M, Kolls J, Oppers-Walgreen B, van den Bersselaar L, Joosten L, Schurr J . Interleukin-17 receptor deficiency results in impaired synovial expression of interleukin-1 and matrix metalloproteinases 3, 9, and 13 and prevents cartilage destruction during chronic reactivated streptococcal cell wall-induced arthritis. Arthritis Rheum. 2005; 52(10):3239-47. DOI: 10.1002/art.21342. View

Vojdani A, Hebroni F, Raphael Y, Erde J, Raxlen B . Novel Diagnosis of Lyme Disease: Potential for CAM Intervention. Evid Based Complement Alternat Med. 2008; 6(3):283-95. PMC: 2722197. DOI: 10.1093/ecam/nem138. View

Wei Y, Li Y, Qiang C . Effects and mechanisms of FR167653, a dual inhibitor of interleukin-1 and tumor necrosis factor, on adjuvant arthritis in rats. Int Immunopharmacol. 2004; 4(13):1625-32. DOI: 10.1016/j.intimp.2004.07.022. View

Zhang G, Lee W, Bausell B, Lao L, Handwerger B, Berman B . Variability in the traditional Chinese medicine (TCM) diagnoses and herbal prescriptions provided by three TCM practitioners for 40 patients with rheumatoid arthritis. J Altern Complement Med. 2005; 11(3):415-21. DOI: 10.1089/acm.2005.11.415. View

Anderton S, van der Zee R, Prakken B, Noordzij A, van Eden W . Activation of T cells recognizing self 60-kD heat shock protein can protect against experimental arthritis. J Exp Med. 1995; 181(3):943-52. PMC: 2191900. DOI: 10.1084/jem.181.3.943. View

10.

Bogdan C . Nitric oxide and the immune response. Nat Immunol. 2001; 2(10):907-16. DOI: 10.1038/ni1001-907. View

11.

Fan A, Lao L, Zhang R, Zhou A, Wang L, Moudgil K . Effects of an acetone extract of Boswellia carterii Birdw. (Burseraceae) gum resin on adjuvant-induced arthritis in lewis rats. J Ethnopharmacol. 2005; 101(1-3):104-9. DOI: 10.1016/j.jep.2005.03.033. View

12.

Kim H, Kim E, cerny J, Moudgil K . Antibody responses to mycobacterial and self heat shock protein 65 in autoimmune arthritis: epitope specificity and implication in pathogenesis. J Immunol. 2006; 177(10):6634-41. DOI: 10.4049/jimmunol.177.10.6634. View

13.

Kuo G, Hawley S, Weiss L, Balkrishnan R, Volk R . Factors associated with herbal use among urban multiethnic primary care patients: a cross-sectional survey. BMC Complement Altern Med. 2004; 4:18. PMC: 539258. DOI: 10.1186/1472-6882-4-18. View

14.

Chu C, Swart D, Alcorn D, Tocker J, Elkon K . Interferon-gamma regulates susceptibility to collagen-induced arthritis through suppression of interleukin-17. Arthritis Rheum. 2007; 56(4):1145-51. DOI: 10.1002/art.22453. View

15.

Crawford N, Cincotta D, Lim A, Powell C . A cross-sectional survey of complementary and alternative medicine use by children and adolescents attending the University Hospital of Wales. BMC Complement Altern Med. 2006; 6:16. PMC: 1475886. DOI: 10.1186/1472-6882-6-16. View

16.

Sengupta K, Alluri K, Satish A, Mishra S, Golakoti T, Sarma K . A double blind, randomized, placebo controlled study of the efficacy and safety of 5-Loxin for treatment of osteoarthritis of the knee. Arthritis Res Ther. 2008; 10(4):R85. PMC: 2575633. DOI: 10.1186/ar2461. View

17.

Durai M, Kim H, Moudgil K . The regulatory C-terminal determinants within mycobacterial heat shock protein 65 are cryptic and cross-reactive with the dominant self homologs: implications for the pathogenesis of autoimmune arthritis. J Immunol. 2004; 173(1):181-8. DOI: 10.4049/jimmunol.173.1.181. View

18.

Zhang R, Fan A, Zhou A, Moudgil K, Ma Z, Lee D . Extract of the Chinese herbal formula Huo Luo Xiao Ling Dan inhibited adjuvant arthritis in rats. J Ethnopharmacol. 2008; 121(3):366-71. PMC: 2818782. DOI: 10.1016/j.jep.2008.11.018. View

19.

Kiyohara H, Matsumoto T, Yamada H . Combination Effects of Herbs in a Multi-herbal Formula: Expression of Juzen-taiho-to's Immuno-modulatory Activity on the Intestinal Immune System. Evid Based Complement Alternat Med. 2004; 1(1):83-91. PMC: 526207. DOI: 10.1093/ecam/neh004. View

20.

Kiyohara H, Matsumoto T, Yamada H . Lignin-carbohydrate complexes: intestinal immune system modulating ingredients in kampo (Japanese herbal) medicine, juzen-taiho-to. Planta Med. 2000; 66(1):20-4. DOI: 10.1055/s-2000-11116. View