Effects of Creatine and β-guanidinopropionic Acid and Alterations in Creatine Transporter and Creatine Kinases Expression in Acute Seizure and Chronic Epilepsy Models

Overview

Journal BMC Neurosci

Publisher Biomed Central

Specialty Neurology

Date 2010 Oct 29

PMID 20979657

Citations 4

Authors

Dae Won Kim

Seong-Il Yeo

Hea Jin Ryu

Ji-Eun Kim

Hong-Ki Song

Oh-Shin Kwon

Soo Young Choi

Tae-Cheon Kang

Affiliations

Soon will be listed here.

Abstract

Background: In order to confirm the roles of creatine (Cr) in epilepsy, we investigated the anti-convulsive effects of Cr, creatine transporter (CRT) and creatine kinases (CKs) against chemical-induced acute seizure activity and chronic epileptic seizure activity.

Results: Two hr after pilocarpine (PILO)-seizure induction, ubiquitous mitochondrial CK (uMtCK) immunoreactivity was unaltered as compared to control level. However, brain-type cytoplasm CK (BCK) immunoreactivity was decreased to 70% of control level. CRT immunoreactivity was decreased to 60% of control level. Following Cr or Tat-CK treatment, uMtCK or CRT immunoreactivity was unaffected, while BCK immunoreactivity in Cr treated group was increased to 3.6-fold of control levels. β-Guanidinopropionic acid (GPA, a competitive CRT inhibitor) reduced BCK and CRT expression. In addition, Cr and tat-BCK treatment delayed the beginning of seizure activity after PILO injection. However, GPA treatment induced spontaneous seizure activity without PILO treatment. In chronic epilepsy rats, both uMtCK and CRT immunoreactivities were reduced in the hippocampus. In contrast, BCK immunoreactivity was similar to that observed in control animals. Cr-, GPA and tat-BCK treatment could not change EEG.

Conclusion: Cr/CK circuit may play an important role in sustaining or exacerbating acute seizure activity, but not chronic epileptic discharge.

Citing Articles

The Metabolic Profile of Plasma During Epileptogenesis in a Rat Model of Lithium-Pilocarpine-Induced Temporal Lobe Epilepsy.

Antmen F, Matpan E, Dayanc E, Savas E, Eken Y, Acar D Mol Neurobiol. 2025; .

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Creatine supplementation enhances anti-tumor immunity by promoting adenosine triphosphate production in macrophages.

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Differences in the hippocampal frequency of creatine inclusions between the acute and latent phases of pilocarpine model defined using synchrotron radiation-based FTIR microspectroscopy.

Kutorasinska J, Setkowicz Z, Janeczko K, Sandt C, Dumas P, Chwiej J Anal Bioanal Chem. 2013; 405(23):7337-45.

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The effect of the creatine analogue beta-guanidinopropionic acid on energy metabolism: a systematic review.

Oudman I, Clark J, Brewster L PLoS One. 2013; 8(1):e52879.

PMID: 23326362 PMC: 3541392. DOI: 10.1371/journal.pone.0052879.

Synchrotron radiation Fourier-transform infrared and Raman microspectroscopy study showing an increased frequency of creatine inclusions in the rat hippocampal formation following pilocarpine-induced seizures.

Dulinska J, Setkowicz Z, Janeczko K, Sandt C, Dumas P, Uram L Anal Bioanal Chem. 2011; 402(7):2267-74.

PMID: 22038587 PMC: 3281206. DOI: 10.1007/s00216-011-5488-z.

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