Regulation of MprF by Antisense RNA Restores Daptomycin Susceptibility to Daptomycin-resistant Isolates of Staphylococcus Aureus

Overview

Journal Antimicrob Agents Chemother

Publisher American Society for Microbiology

Specialty Pharmacology

Date 2010 Oct 27

PMID 20974866

Citations 28

Authors

Aileen Rubio

Mary Conrad

Robert J Haselbeck

Kedar G C

Vickie Brown-Driver

John Finn

Jared A Silverman

Affiliations

Soon will be listed here.

Abstract

Mutations in mprF have been shown to result in reduced susceptibility to daptomycin and other cationic antibacterials. An mprF antisense-inducible plasmid was constructed and used to demonstrate that depletion of mprF can reestablish susceptibility to daptomycin. Inducing antisense to mprF also resulted in increased susceptibility to vancomycin and gentamicin but, paradoxically, decreased susceptibility to oxacillin. These results suggest that mprF mutations that reduce susceptibility to cationic antibacterials result in a gain-of-function phenotype.

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