» Articles » PMID: 20974479

Intrinsic Phospholipase A2 Activity of Adeno-associated Virus is Involved in Endosomal Escape of Incoming Particles

Overview
Journal Virology
Specialty Microbiology
Date 2010 Oct 27
PMID 20974479
Citations 50
Authors
Affiliations
Soon will be listed here.
Abstract

The unique region of the VP1 capsid protein of adeno-associated viruses (AAV) in common with autonomously replicating parvoviruses comprises a secreted phospholipase A2 (sPLA2) homology domain. While the sPLA2 domain of Minute Virus of Mice has recently been shown to mediate endosomal escape by lipolytic pore formation, experimental evidence for a similar function in AAV infection is still lacking. Here, we explored the function of the sPLA2 domain of AAV by making use of the serotype 2 mutant (76)HD/AN. The sPLA2 defect in (76)HD/AN, which severely impairs AAV's infectivity, could be complemented in trans by co-infection with wild-type AAV2. Furthermore, co-infection with endosomolytically active, but not with inactive adenoviral variants partially rescued (76)HD/AN, providing the first evidence for a function of this domain in endosomal escape of incoming AAV particles.

Citing Articles

Progress in AAV-Mediated In Vivo Gene Therapy and Its Applications in Central Nervous System Diseases.

Wang S, Xiao L Int J Mol Sci. 2025; 26(5).

PMID: 40076831 PMC: 11899905. DOI: 10.3390/ijms26052213.


Structural basis for nuclear import of adeno-associated virus serotype 6 capsid protein.

Hoad M, Nematollahzadeh S, Petersen G, Roby J, Alvisi G, Forwood J J Virol. 2024; 99(1):e0134524.

PMID: 39692478 PMC: 11784021. DOI: 10.1128/jvi.01345-24.


Adeno-associated virus 9 (AAV9) viral proteins VP1, VP2, and membrane-associated accessory protein (MAAP) differentially influence transgene expression.

Powell S, McCown T J Virol. 2024; 98(11):e0168124.

PMID: 39475275 PMC: 11575147. DOI: 10.1128/jvi.01681-24.


The Expression and Function of the Small Nonstructural Proteins of Adeno-Associated Viruses (AAVs).

Kuz C, McFarlin S, Qiu J Viruses. 2024; 16(8).

PMID: 39205189 PMC: 11359079. DOI: 10.3390/v16081215.


Therapeutic Application and Structural Features of Adeno-Associated Virus Vector.

Matsuzaka Y, Yashiro R Curr Issues Mol Biol. 2024; 46(8):8464-8498.

PMID: 39194716 PMC: 11353222. DOI: 10.3390/cimb46080499.