Infusion of Mature Megakaryocytes into Mice Yields Functional Platelets

Overview

Journal J Clin Invest

Specialty General Medicine

Date 2010 Oct 26

PMID 20972336

Citations 63

Authors

Rudy Fuentes

Yuhuan Wang

Jessica Hirsch

Cheng Wang

Lubica Rauova

G Scott Worthen

M Anna Kowalska

Mortimer Poncz

Affiliations

Soon will be listed here.

Abstract

Thrombopoiesis, the process by which circulating platelets arise from megakaryocytes, remains incompletely understood. Prior studies suggest that megakaryocytes shed platelets in the pulmonary vasculature. To better understand thrombopoiesis and to develop a potential platelet transfusion strategy that is not dependent upon donors, of which there remains a shortage, we examined whether megakaryocytes infused into mice shed platelets. Infused megakaryocytes led to clinically relevant increases in platelet numbers. The released platelets were normal in size, displayed appropriate surface markers, and had a near-normal circulating half-life. The functionality of the donor-derived platelets was also demonstrated in vivo. The infused megakaryocytes mostly localized to the pulmonary vasculature, where they appeared to shed platelets. These data suggest that it may be unnecessary to generate platelets from ex vivo grown megakaryocytes to achieve clinically relevant increases in platelet numbers.

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