Genetic and Biochemical Analysis of the SLN1 Pathway in Saccharomyces Cerevisiae

Overview

Journal Methods Enzymol

Specialty Biochemistry

Date 2010 Oct 16

PMID 20946854

Citations 12

Authors

Jan S Fassler

Ann H West

Affiliations

Soon will be listed here.

Abstract

The histidine kinase-based signal transduction pathway was first uncovered in bacteria and is a prominent form of regulation in prokaryotes. However, this type of signal transduction is not unique to prokaryotes; over the last decade two-component signal transduction pathways have been identified and characterized in diverse eukaryotes, from unicellular yeasts to multicellular land plants. A number of small but important differences have been noted in the architecture and function of eukaryotic pathways. Because of the powerful genetic approaches and facile molecular analysis associated with the yeast system, the SLN1 osmotic response pathway in Saccharomyces cerevisiae is particularly useful as a eukaryotic pathway model. This chapter provides an overview of genetic and biochemical methods that have been important in elucidating the stimulus-response events that underlie this pathway and in understanding the details of a eukaryotic His-Asp phosphorelay.

Citing Articles

Function of the pseudo phosphotransfer proteins has diverged between rice and Arabidopsis.

Vaughan-Hirsch J, Tallerday E, Burr C, Hodgens C, Boeshore S, Beaver K Plant J. 2021; 106(1):159-173.

PMID: 33421204 PMC: 11497342. DOI: 10.1111/tpj.15156.

Transcriptome Analysis of Dimorphic Fungus Sporothrix schenckii Exposed to Temperature Stress.

He D, Zhang X, Gao S, You H, Zhao Y, Wang L Int Microbiol. 2020; 24(1):25-35.

PMID: 32691258 PMC: 7873001. DOI: 10.1007/s10123-020-00136-y.

Role of the highly conserved G68 residue in the yeast phosphorelay protein Ypd1: implications for interactions between histidine phosphotransfer (HPt) and response regulator proteins.

Kennedy E, Hebdon S, Menon S, Foster C, Copeland D, Xu Q BMC Biochem. 2019; 20(1):1.

PMID: 30665347 PMC: 6341664. DOI: 10.1186/s12858-019-0104-5.

Design, characterization and in vivo functioning of a light-dependent histidine protein kinase in the yeast Saccharomyces cerevisiae.

Bury A, Hellingwerf K AMB Express. 2018; 8(1):53.

PMID: 29611000 PMC: 5880792. DOI: 10.1186/s13568-018-0582-7.

The yeasts phosphorelay systems: a comparative view.

Salas-Delgado G, Ongay-Larios L, Kawasaki-Watanabe L, Lopez-Villasenor I, Coria R World J Microbiol Biotechnol. 2017; 33(6):111.

PMID: 28470426 DOI: 10.1007/s11274-017-2272-z.

References

Tao W, Deschenes R, Fassler J . Intracellular glycerol levels modulate the activity of Sln1p, a Saccharomyces cerevisiae two-component regulator. J Biol Chem. 1998; 274(1):360-7. PMC: 2909977. DOI: 10.1074/jbc.274.1.360. View

Lawrence C . Classical mutagenesis techniques. Methods Enzymol. 1991; 194:273-81. DOI: 10.1016/0076-6879(91)94021-4. View

Albertyn J, Hohmann S, Prior B . Characterization of the osmotic-stress response in Saccharomyces cerevisiae: osmotic stress and glucose repression regulate glycerol-3-phosphate dehydrogenase independently. Curr Genet. 1994; 25(1):12-8. DOI: 10.1007/BF00712960. View

Ault A, Fassler J, Deschenes R . Altered phosphotransfer in an activated mutant of the Saccharomyces cerevisiae two-component osmosensor Sln1p. Eukaryot Cell. 2002; 1(2):174-80. PMC: 118030. DOI: 10.1128/EC.1.2.174-180.2002. View

Tao W, Malone C, Ault A, Deschenes R, Fassler J . A cytoplasmic coiled-coil domain is required for histidine kinase activity of the yeast osmosensor, SLN1. Mol Microbiol. 2002; 43(2):459-73. PMC: 2892222. DOI: 10.1046/j.1365-2958.2002.02757.x. View

Ota I, Varshavsky A . A gene encoding a putative tyrosine phosphatase suppresses lethality of an N-end rule-dependent mutant. Proc Natl Acad Sci U S A. 1992; 89(6):2355-9. PMC: 48656. DOI: 10.1073/pnas.89.6.2355. View

Fassler J, Gray W, Malone C, Tao W, Lin H, Deschenes R . Activated alleles of yeast SLN1 increase Mcm1-dependent reporter gene expression and diminish signaling through the Hog1 osmosensing pathway. J Biol Chem. 1997; 272(20):13365-71. DOI: 10.1074/jbc.272.20.13365. View

Posas F, Maeda T, Witten E, Thai T, Saito H . Yeast HOG1 MAP kinase cascade is regulated by a multistep phosphorelay mechanism in the SLN1-YPD1-SSK1 "two-component" osmosensor. Cell. 1996; 86(6):865-75. DOI: 10.1016/s0092-8674(00)80162-2. View

West A . Functional roles of conserved amino acid residues surrounding the phosphorylatable histidine of the yeast phosphorelay protein YPD1. Mol Microbiol. 2000; 37(1):136-44. DOI: 10.1046/j.1365-2958.2000.01973.x. View

10.

Ostrander D, Gorman J . The extracellular domain of the Saccharomyces cerevisiae Sln1p membrane osmolarity sensor is necessary for kinase activity. J Bacteriol. 1999; 181(8):2527-34. PMC: 93681. DOI: 10.1128/JB.181.8.2527-2534.1999. View

11.

Marina A, Waldburger C, Hendrickson W . Structure of the entire cytoplasmic portion of a sensor histidine-kinase protein. EMBO J. 2005; 24(24):4247-59. PMC: 1356327. DOI: 10.1038/sj.emboj.7600886. View

12.

Georgieva B, Rothstein R . Kar-mediated plasmid transfer between yeast strains: alternative to traditional transformation methods. Methods Enzymol. 2002; 350:278-89. DOI: 10.1016/s0076-6879(02)50969-1. View

13.

Maeda T, Takekawa M, Saito H . Activation of yeast PBS2 MAPKK by MAPKKKs or by binding of an SH3-containing osmosensor. Science. 1995; 269(5223):554-8. DOI: 10.1126/science.7624781. View

14.

Singh M, Berger B, Kim P, Berger J, Cochran A . Computational learning reveals coiled coil-like motifs in histidine kinase linker domains. Proc Natl Acad Sci U S A. 1998; 95(6):2738-43. PMC: 19638. DOI: 10.1073/pnas.95.6.2738. View

15.

Xu Q, Porter S, West A . The yeast YPD1/SLN1 complex: insights into molecular recognition in two-component signaling systems. Structure. 2003; 11(12):1569-81. DOI: 10.1016/j.str.2003.10.016. View

16.

Luyten K, Albertyn J, Skibbe W, Prior B, Ramos J, Thevelein J . Fps1, a yeast member of the MIP family of channel proteins, is a facilitator for glycerol uptake and efflux and is inactive under osmotic stress. EMBO J. 1995; 14(7):1360-71. PMC: 398221. DOI: 10.1002/j.1460-2075.1995.tb07122.x. View

17.

Sparling D, West A . Novel role for an HPt domain in stabilizing the phosphorylated state of a response regulator domain. J Bacteriol. 2000; 182(23):6673-8. PMC: 111409. DOI: 10.1128/JB.182.23.6673-6678.2000. View

18.

Li S, Ault A, Malone C, Raitt D, Dean S, Johnston L . The yeast histidine protein kinase, Sln1p, mediates phosphotransfer to two response regulators, Ssk1p and Skn7p. EMBO J. 1998; 17(23):6952-62. PMC: 1171043. DOI: 10.1093/emboj/17.23.6952. View

19.

Maeda T, Witten E, Saito H . Regulation of the Saccharomyces cerevisiae HOG1 mitogen-activated protein kinase by the PTP2 and PTP3 protein tyrosine phosphatases. Mol Cell Biol. 1997; 17(3):1289-97. PMC: 231854. DOI: 10.1128/MCB.17.3.1289. View

20.

Li S, Dean S, Li Z, Horecka J, Deschenes R, Fassler J . The eukaryotic two-component histidine kinase Sln1p regulates OCH1 via the transcription factor, Skn7p. Mol Biol Cell. 2002; 13(2):412-24. PMC: 65637. DOI: 10.1091/mbc.01-09-0434. View