Functional Divergence of Poplar Histidine-Aspartate Kinase HK1 Paralogs in Response to Osmotic Stress

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2016 Dec 13

PMID 27941652

Citations 9

Authors

Francois Hericourt

Francoise Chefdor

Ines Djeghdir

Melanie Larcher

Florent Lafontaine

Vincent Courdavault

Daniel Auguin

Franck Coste

Christiane Depierreux

Mirai Tanigawa

Tatsuya Maeda

Gaelle Glevarec

Sabine Carpin

Affiliations

Soon will be listed here.

Abstract

Previous works have shown the existence of protein partnerships belonging to a MultiStep Phosphorelay (MSP) in putatively involved in osmosensing. This study is focused on the identification of a histidine-aspartate kinase, HK1b, paralog of HK1a. The characterization of HK1b showed its ability to homo- and hetero-dimerize and to interact with a few Histidine-containing Phosphotransfer (HPt) proteins, suggesting a preferential partnership in poplar MSP linked to drought perception. Furthermore, determinants for interaction specificity between HK1a/1b and HPts were studied by mutagenesis analysis, identifying amino acids involved in this specificity. The expression analysis in different poplar organs revealed its co-expression with three , reinforcing the hypothesis of partnership participation in the MSP in planta. Moreover, HK1b was shown to act as an osmosensor with kinase activity in a functional complementation assay of an osmosensor deficient yeast strain. These results revealed that HK1b showed a different behaviour for canonical phosphorylation of histidine and aspartate residues. These phosphorylation modularities of canonical amino acids could explain the improved osmosensor performances observed in yeast. As conserved duplicates reflect the selective pressures imposed by the environmental requirements on the species, our results emphasize the importance of gene duplication in poplar adaptation to drought stress.

Citing Articles

Searching for Osmosensing Determinants in Poplar Histidine-Aspartate Kinases.

Makhokh H, Lafite P, Larcher M, Lamblin F, Chefdor F, Depierreux C Int J Mol Sci. 2023; 24(7).

PMID: 37047295 PMC: 10093795. DOI: 10.3390/ijms24076318.

Proteomics approach to investigating osmotic stress effects on pistachio.

Pakzad R, Fatehi F, Kalantar M, Maleki M Front Plant Sci. 2023; 13:1041649.

PMID: 36762186 PMC: 9907329. DOI: 10.3389/fpls.2022.1041649.

Cytokinin Signaling Downstream of the His-Asp Phosphorelay Network: Cytokinin-Regulated Genes and Their Functions.

Kroll C, Brenner W Front Plant Sci. 2020; 11:604489.

PMID: 33329676 PMC: 7718014. DOI: 10.3389/fpls.2020.604489.

What do archaeal and eukaryotic histidine kinases sense?.

Papon N, Stock A F1000Res. 2020; 8.

PMID: 31942238 PMC: 6944256. DOI: 10.12688/f1000research.20094.1.

New Insight into HPts as Hubs in Poplar Cytokinin and Osmosensing Multistep Phosphorelays: Cytokinin Pathway Uses Specific HPts.

Hericourt F, Larcher M, Chefdor F, Koudounas K, Carqueijeiro I, Lemos Cruz P Plants (Basel). 2019; 8(12).

PMID: 31835814 PMC: 6963366. DOI: 10.3390/plants8120591.

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