Genome-wide Mapping of Nucleosomes in Yeast

Overview

Journal Methods Enzymol

Specialty Biochemistry

Date 2010 Oct 16

PMID 20946808

Citations 24

Authors

Oliver J Rando

Affiliations

Soon will be listed here.

Abstract

The packaging of eukaryotic genomes into chromatin has wide-ranging influences on all DNA-templated processes, from DNA repair to transcriptional regulation. The repeating subunit of chromatin is the nucleosome, which comprises 147 bp of DNA wrapped around an octamer of proteins. Positioning of nucleosomes relative to underlying DNA is a key factor in the regulation of gene transcription by chromatin, as DNA sequences between nucleosomes are more accessible to regulatory factors than are DNA sequences within nucleosomes. Here, I describe protocols for mapping nucleosome positions across the yeast genome.

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