Microbial Diversity of Supra- and Subgingival Biofilms on Freshly Colonized Titanium Implant Abutments in the Human Mouth

Overview

Journal Eur J Clin Microbiol Infect Dis

Publisher Springer

Specialties Infectious Diseases
Microbiology

Date 2010 Oct 9

PMID 20931254

Citations 9

Authors

W Heuer

M Stiesch

W R Abraham

Affiliations

Soon will be listed here.

Abstract

Supra- and subgingival biofilm formation is considered to be mainly responsible for early implant failure caused by inflammations of periimplant tissues. Nevertheless, little is known about the complex microbial diversity and interindividual similarities around dental implants. An atraumatic assessment was made of the diversity of microbial communities around titanium implants by single strand conformation polymorphism (SSCP) analysis of the 16S rRNA gene amplicons as well as subsequent sequence analysis. Samples of adherent supra- and subgingival periimplant biofilms were collected from ten patients. Additionally, samples of sulcusfluid were taken at titanium implant abutments and remaining teeth. The bacteria in the samples were characterized by SSCP and sequence analysis. A high diversity of bacteria varying between patients and within one patient at different locations was found. Bacteria characteristic for sulcusfluid and supra- and subgingival biofilm communities were identified. Sulcusfluid of the abutments showed higher abundance of Streptococcus species than from residual teeth. Prevotella and Rothia species frequently reported from the oral cavity were not detected at the abutments suggesting a role as late colonizers. Different niches in the human mouth are characterized by specific groups of bacteria. Implant abutments are a very valuable approach to study dental biofilm development in vivo.

Citing Articles

Structure and composition of early biofilms formed on dental implants are complex, diverse, subject-specific and dynamic.

Dieckow S, Szafranski S, Grischke J, Qu T, Doll-Nikutta K, Steglich M NPJ Biofilms Microbiomes. 2024; 10(1):155.

PMID: 39719447 PMC: 11668855. DOI: 10.1038/s41522-024-00624-3.

Pilot Study on the Use of a Laser-Structured Double Diamond Electrode (DDE) for Biofilm Removal from Dental Implant Surfaces.

Koch M, Burkovski A, Zulla M, Rosiwal S, Geissdorfer W, Dittmar R J Clin Med. 2020; 9(9).

PMID: 32967183 PMC: 7565428. DOI: 10.3390/jcm9093036.

Biofilm Removal and Bacterial Re-Colonization Inhibition of a Novel Erythritol/Chlorhexidine Air-Polishing Powder on Titanium Disks.

Mensi M, Cochis A, Sordillo A, Uberti F, Rimondini L Materials (Basel). 2018; 11(9).

PMID: 30142888 PMC: 6164901. DOI: 10.3390/ma11091510.

Bacterial Colonization and Tissue Compatibility of Denture Base Resins.

Olms C, Yahiaoui-Doktor M, Remmerbach T, Stingu C Dent J (Basel). 2018; 6(2).

PMID: 29914101 PMC: 6023505. DOI: 10.3390/dj6020020.

The Peri-Implant and Periodontal Microbiota in Patients with and without Clinical Signs of Inflammation.

Jakobi M, Stumpp S, Stiesch M, Eberhard J, Heuer W Dent J (Basel). 2018; 3(2):24-42.

PMID: 29567923 PMC: 5851201. DOI: 10.3390/dj3020024.

References

Paster B, Boches S, Galvin J, Ericson R, Lau C, Levanos V . Bacterial diversity in human subgingival plaque. J Bacteriol. 2001; 183(12):3770-83. PMC: 95255. DOI: 10.1128/JB.183.12.3770-3783.2001. View

Elter C, Heuer W, Demling A, Hannig M, Heidenblut T, Bach F . Supra- and subgingival biofilm formation on implant abutments with different surface characteristics. Int J Oral Maxillofac Implants. 2008; 23(2):327-34. View

Heuer W, Elter C, Demling A, Neumann A, Suerbaum S, Hannig M . Analysis of early biofilm formation on oral implants in man. J Oral Rehabil. 2007; 34(5):377-82. DOI: 10.1111/j.1365-2842.2007.01725.x. View

Slots J . Microflora in the healthy gingival sulcus in man. Scand J Dent Res. 1977; 85(4):247-54. DOI: 10.1111/j.1600-0722.1977.tb00560.x. View

Scannapieco F . Role of oral bacteria in respiratory infection. J Periodontol. 1999; 70(7):793-802. DOI: 10.1902/jop.1999.70.7.793. View

Hughes C, ANDERSEN R, Kolenbrander P . Characterization of Veillonella atypica PK1910 adhesin-mediated coaggregation with oral Streptococcus spp. Infect Immun. 1992; 60(3):1178-86. PMC: 257610. DOI: 10.1128/iai.60.3.1178-1186.1992. View

Socransky S, Haffajee A . The bacterial etiology of destructive periodontal disease: current concepts. J Periodontol. 1992; 63(4 Suppl):322-31. DOI: 10.1902/jop.1992.63.4s.322. View

Marchandin H, Teyssier C, de Buochberg M, Jean-Pierre H, Carriere C, Jumas-Bilak E . Intra-chromosomal heterogeneity between the four 16S rRNA gene copies in the genus Veillonella: implications for phylogeny and taxonomy. Microbiology (Reading). 2003; 149(Pt 6):1493-1501. DOI: 10.1099/mic.0.26132-0. View

Slots J, Rams T . New views on periodontal microbiota in special patient categories. J Clin Periodontol. 1991; 18(6):411-20. DOI: 10.1111/j.1600-051x.1991.tb02309.x. View

10.

Ericsson I, Berglundh T, Marinello C, Liljenberg B, Lindhe J . Long-standing plaque and gingivitis at implants and teeth in the dog. Clin Oral Implants Res. 1992; 3(3):99-103. DOI: 10.1034/j.1600-0501.1992.030301.x. View

11.

Bik E, Long C, Armitage G, Loomer P, Emerson J, Mongodin E . Bacterial diversity in the oral cavity of 10 healthy individuals. ISME J. 2010; 4(8):962-74. PMC: 2941673. DOI: 10.1038/ismej.2010.30. View

12.

Zaura E, Keijser B, Huse S, Crielaard W . Defining the healthy "core microbiome" of oral microbial communities. BMC Microbiol. 2009; 9:259. PMC: 2805672. DOI: 10.1186/1471-2180-9-259. View

13.

Li Y, Ku C, Xu J, Saxena D, Caufield P . Survey of oral microbial diversity using PCR-based denaturing gradient gel electrophoresis. J Dent Res. 2005; 84(6):559-64. DOI: 10.1177/154405910508400614. View

14.

Mikx F, van der Hoeven J . Symbiosis of Streptococcus mutans and Veillonella alcalescens in mixed continuous cultures. Arch Oral Biol. 1975; 20(7):407-10. DOI: 10.1016/0003-9969(75)90224-1. View

15.

Willcox M, Drucker D, Green R . Relative cariogenicity and in-vivo plaque-forming ability of the bacterium Streptococcus oralis in gnotobiotic WAG/RIJ rats. Arch Oral Biol. 1987; 32(6):455-7. DOI: 10.1016/0003-9969(87)90083-5. View

16.

Berglundh T, Lindhe J, Marinello C, Ericsson I, Liljenberg B . Soft tissue reaction to de novo plaque formation on implants and teeth. An experimental study in the dog. Clin Oral Implants Res. 1992; 3(1):1-8. DOI: 10.1034/j.1600-0501.1992.030101.x. View

17.

Loe H, THEILADE E, Jensen S . EXPERIMENTAL GINGIVITIS IN MAN. J Periodontol (1930). 1965; 36:177-87. DOI: 10.1902/jop.1965.36.3.177. View

18.

Kroes I, Lepp P, Relman D . Bacterial diversity within the human subgingival crevice. Proc Natl Acad Sci U S A. 1999; 96(25):14547-52. PMC: 24473. DOI: 10.1073/pnas.96.25.14547. View

19.

Socransky S, Haffajee A, Cugini M, Smith C, Kent Jr R . Microbial complexes in subgingival plaque. J Clin Periodontol. 1998; 25(2):134-44. DOI: 10.1111/j.1600-051x.1998.tb02419.x. View

20.

Zijnge V, Harmsen H, Kleinfelder J, van der Rest M, Degener J, Welling G . Denaturing gradient gel electrophoresis analysis to study bacterial community structure in pockets of periodontitis patients. Oral Microbiol Immunol. 2003; 18(1):59-65. DOI: 10.1034/j.1399-302x.2003.180110.x. View