Bacterial Colonization and Tissue Compatibility of Denture Base Resins

Overview

Journal Dent J (Basel)

Specialty Dentistry

Date 2018 Jun 20

PMID 29914101

Citations 5

Authors

Constanze Olms

Maryam Yahiaoui-Doktor

Torsten W Remmerbach

Catalina Suzana Stingu

Affiliations

Soon will be listed here.

Abstract

Currently, there is minimal clinical data regarding biofilm composition on the surface of denture bases and the clinical tissue compatibility. Therefore, the aim of this experimental study was to compare the bacterial colonization and the tissue compatibility of a hypoallergenic polyamide with a frequently used PMMA resin tested intraorally in a randomized split-mouth design. Test specimens made of polyamide ( = 10) and PMMA ( = 10) were attached over a molar band appliance in oral cavity of 10 subjects. A cytological smear test was done from palatal mucosa at baseline and after four weeks. The monolayers were inspected for micronuclei. After four weeks in situ, the appliance was removed. The test specimens were immediately cultivated on non-selective and selective nutrient media. All growing colonies were identified using VITEK-MS. The anonymized results were analyzed descriptively. A total of 110 different bacterial species could be isolated, including putative pathogens. An average of 17.8 different bacterial species grew on the PMMA specimens, and 17.3 on the polyamide specimens. The highest number of different bacterial species was = 24, found on a PMMA specimen. On the two specimens, a similar bacterial distribution was observed. Micronuclei, as a marker for genotoxic potential of dental materials, were not detected. This study indicates that the composition of bacterial biofilm developed on these resins after four weeks is not influenced by the type of resin itself. The two materials showed no cytological differences. This investigation suggests that polyamide and PMMA are suitable for clinical use as denture base material.

Citing Articles

Different Polymers for the Base of Removable Dentures? Part II: A Narrative Review of the Dynamics of Microbial Plaque Formation on Dentures.

Le Bars P, Kouadio A, Amouriq Y, Bodic F, Blery P, Bandiaky O Polymers (Basel). 2024; 16(1).

PMID: 38201705 PMC: 10780608. DOI: 10.3390/polym16010040.

Long-term application of silver nanoparticles in dental restoration materials: potential toxic injury to the CNS.

Wang K, Wang S, Yin J, Yang Q, Yu Y, Chen L J Mater Sci Mater Med. 2023; 34(11):52.

PMID: 37855967 PMC: 10587321. DOI: 10.1007/s10856-023-06753-z.

Characterization of the Oral Microbiome in Wearers of Fixed and Removable Implant or Non-Implant-Supported Prostheses in Healthy and Pathological Oral Conditions: A Narrative Review.

DAmbrosio F, Santella B, Di Palo M, Giordano F, Lo Giudice R Microorganisms. 2023; 11(4).

PMID: 37110463 PMC: 10145620. DOI: 10.3390/microorganisms11041041.

The denture microbiome in health and disease: an exploration of a unique community.

Redfern J, Tosheva L, Malic S, Butcher M, Ramage G, Verran J Lett Appl Microbiol. 2022; 75(2):195-209.

PMID: 35634756 PMC: 9546486. DOI: 10.1111/lam.13751.

Heat-cured poly(methyl methacrylate) resin incorporated with different food preservatives as an anti-microbial denture base material.

Ratanajanchai M, Kanchanavasita W, Suputtamongkol K, Wonglamsam A, Thamapipol S, Sae-Khow O J Dent Sci. 2021; 16(2):706-712.

PMID: 33854722 PMC: 8025184. DOI: 10.1016/j.jds.2020.09.006.

References

Bigatti M, Lamberti L, Rizzi F, Cannas M, Allasia G . In vitro micronucleus induction by polymethyl methacrylate bone cement in cultured human lymphocytes. Mutat Res. 1994; 321(3):133-7. DOI: 10.1016/0165-1218(94)90037-x. View

Abuzar M, Bellur S, Duong N, Kim B, Lu P, Palfreyman N . Evaluating surface roughness of a polyamide denture base material in comparison with poly (methyl methacrylate). J Oral Sci. 2011; 52(4):577-81. DOI: 10.2334/josnusd.52.577. View

Zaremba M, Daniluk T, Rozkiewicz D, Cylwik-Rokicka D, Kierklo A, Tokajuk G . Incidence rate of Candida species in the oral cavity of middle-aged and elderly subjects. Adv Med Sci. 2007; 51 Suppl 1:233-6. View

Hahnel S, Schwarz S, Zeman F, Schafer L, Behr M . Prevalence of xerostomia and hyposalivation and their association with quality of life in elderly patients in dependence on dental status and prosthetic rehabilitation: a pilot study. J Dent. 2014; 42(6):664-70. DOI: 10.1016/j.jdent.2014.03.003. View

Sachdeo A, Haffajee A, Socransky S . Biofilms in the edentulous oral cavity. J Prosthodont. 2008; 17(5):348-56. DOI: 10.1111/j.1532-849X.2008.00301.x. View

Singh K, Aeran H, Kumar N, Gupta N . Flexible thermoplastic denture base materials for aesthetical removable partial denture framework. J Clin Diagn Res. 2013; 7(10):2372-3. PMC: 3843478. DOI: 10.7860/JCDR/2013/5020.3527. View

Waltimo T, Vallittu P, Haapasalo M . Adherence of Candida species to newly polymerized and water-stored denture base polymers. Int J Prosthodont. 2002; 14(5):457-60. View

Fuchslocher Hellemann C, Grade S, Heuer W, Dittmer M, Stiesch M, Schwestka-Polly R . Three-dimensional analysis of initial biofilm formation on polytetrafluoroethylene in the oral cavity. J Orofac Orthop. 2013; 74(6):458-67. DOI: 10.1007/s00056-013-0174-8. View

Bourgeois D, David A, Inquimbert C, Tramini P, Molinari N, Carrouel F . Quantification of carious pathogens in the interdental microbiota of young caries-free adults. PLoS One. 2017; 12(10):e0185804. PMC: 5634565. DOI: 10.1371/journal.pone.0185804. View

10.

Remmerbach T, Pomjanski N, Bauer U, Neumann H . Liquid-based versus conventional cytology of oral brush biopsies: a split-sample pilot study. Clin Oral Investig. 2017; 21(8):2493-2498. DOI: 10.1007/s00784-017-2047-9. View

11.

Gendron R, Grenier D . The oral cavity as a reservoir of bacterial pathogens for focal infections. Microbes Infect. 2000; 2(8):897-906. DOI: 10.1016/s1286-4579(00)00391-9. View

12.

Grover S, Mujib A, Jahagirdar A, Telagi N, Kulkarni P . A comparative study for selectivity of micronuclei in oral exfoliated epithelial cells. J Cytol. 2013; 29(4):230-5. PMC: 3543590. DOI: 10.4103/0970-9371.103940. View

13.

Bremer F, Grade S, Kohorst P, Stiesch M . In vivo biofilm formation on different dental ceramics. Quintessence Int. 2011; 42(7):565-74. View

14.

Moalic E, Gestalin A, Quinio D, Gest P, Zerilli A, Le Flohic A . The extent of oral fungal flora in 353 students and possible relationships with dental caries. Caries Res. 2001; 35(2):149-55. DOI: 10.1159/000047447. View

15.

Kawara M, Iwata Y, Iwasaki M, Komoda Y, Iida T, Asano T . Scratch test of thermoplastic denture base resins for non-metal clasp dentures. J Prosthodont Res. 2013; 58(1):35-40. DOI: 10.1016/j.jpor.2013.10.002. View

16.

Hayama F, Motta A, Silva A, Migliari D . Liquid-based preparations versus conventional cytology: specimen adequacy and diagnostic agreement in oral lesions. Med Oral Patol Oral Cir Bucal. 2005; 10(2):115-22. View

17.

Majchrzak K, Mierzwinska-Nastalska E, Chmura A, Kwiatkowski A, Paczek L, Mlynarczyk G . Clinical and Microbiological Evaluation of Removable Prosthetic Restorations in Patients Treated With an Organ Transplant. Transplant Proc. 2016; 48(5):1418-22. DOI: 10.1016/j.transproceed.2016.01.052. View

18.

Belien J, Copper M, Braakhuis B, Snow G, Baak J . Standardization of counting micronuclei: definition of a protocol to measure genotoxic damage in human exfoliated cells. Carcinogenesis. 1995; 16(10):2395-400. DOI: 10.1093/carcin/16.10.2395. View

19.

Kedjarune U, Charoenworaluk N, Koontongkaew S . Release of methyl methacrylate from heat-cured and autopolymerized resins: cytotoxicity testing related to residual monomer. Aust Dent J. 1999; 44(1):25-30. DOI: 10.1111/j.1834-7819.1999.tb00532.x. View

20.

Olms C, Hix N, Neumann H, Yahiaoui-Doktor M, Remmerbach T . Clinical comparison of liquid-based and conventional cytology of oral brush biopsies: a randomized controlled trial. Head Face Med. 2018; 14(1):9. PMC: 5975412. DOI: 10.1186/s13005-018-0166-4. View