Association of Pre-microRNAs Genetic Variants with Susceptibility in Systemic Lupus Erythematosus

Overview

Journal Mol Biol Rep

Specialty Molecular Biology

Date 2010 Sep 17

PMID 20845080

Citations 22

Authors

Junlong Zhang

Bin Yang

Binwu Ying

Dongdong Li

Yunying Shi

Xingbo Song

Bei Cai

Zhuochun Huang

Yongkang Wu

Lanlan Wang

Affiliations

Soon will be listed here.

Abstract

MicroRNAs (miRNAs) may play important roles in SLE, but genetic polymorphisms of miRNAs and their relationships with various autoantibodies present in SLE patients remain unclear. Here, we report that 213 SLE patients and 209 healthy individuals of Chinese had been taken into this case-control studies, which had been performed by selecting two miRNAs (hsa-mir-146a rs2910164 G>C, and hsa-mir-499 rs3746444 T>C) to analyze the genetic polymorphisms. The single nucleotide polymorphism (SNP) variants had been analyzed by PCR-RFLP and serum anti-ribonucleoprotein (anti-RNP), anti-Sm nuclear antigen (anti-Sm) antibodies had been determined by an anti-ENA kit and serum anti-double-stranded DNA (anti-dsDNA) antibodies had been assessed by indirect immunofluorescence. We found that hsa-mir-146a rs2910164 and hsa-mir-499 rs3746444 polymorphisms had no significant relationship with SLE susceptibility. The genotype frequencies of rs2910164 (GG, CC, and GC) were 16, 37, and 47% in SLE patients, but 11, 39, and 50% in healthy group (P = 0.397), respectively; The genotype frequencies of rs3746444 (CC, TT, and TC) were 3, 74, and 23% in SLE patients, but 3, 76, and 22% in healthy group (P = 0.892), respectively. The G and C allele frequencies of rs2910164 were 39 and 61% in SLE patients, but 36 and 64% in healthy group (P = 0.990), respectively. The C and T allele frequencies of rs3746444 were 15 and 85% in SLE patients, but 14 and 86% in healthy group (P = 0.702), respectively. In addition, we also showed no significant difference in the distribution of rs2910164 and rs3746444 genotypes in each of the three antibodies (anti-RNP, anti-Sm, and anti-dsDNA).

Citing Articles

Association between Susceptibility to Systemic Lupus Erythematous and Polymorphisms in miR-146a and miR-499: A Meta-Analysis.

Lee Y, Song G Int Arch Allergy Immunol. 2023; 184(8):814-821.

PMID: 37231940 PMC: 10413798. DOI: 10.1159/000527575.

mir-146a genetic polymorphisms in systemic lupus erythematosus patients: Correlation with disease manifestations.

El-Akhras B, Ali Y, El-Masry S, Bassyouni I, El-Sayed I, Talaat R Noncoding RNA Res. 2022; 7(3):142-149.

PMID: 35756164 PMC: 9192690. DOI: 10.1016/j.ncrna.2022.05.001.

Association between miRNA-499 gene polymorphism and autoimmune diseases: A meta-analysis.

Kong X, Diao S, Xu H, Sun J, Ma B PLoS One. 2022; 17(3):e0266265.

PMID: 35358276 PMC: 8970500. DOI: 10.1371/journal.pone.0266265.

Meta-Analysis of miRNA Variants Associated with Susceptibility to Autoimmune Disease.

Zhang J, Tan H, Cao Q, Su G, Yang P Dis Markers. 2021; 2021:9978460.

PMID: 34659590 PMC: 8519726. DOI: 10.1155/2021/9978460.

Functional polymorphisms in and are associated with systemic lupus erythematosus but not with rheumatoid arthritis or Graves' disease in Mexican patients.

Aleman-Avila I, Jimenez-Morales M, Beltran-Ramirez O, Barbosa-Cobos R, Jimenez-Morales S, Sanchez-Munoz F Oncotarget. 2017; 8(54):91876-91886.

PMID: 29190882 PMC: 5696148. DOI: 10.18632/oncotarget.19621.

References

Harfe B . MicroRNAs in vertebrate development. Curr Opin Genet Dev. 2005; 15(4):410-5. DOI: 10.1016/j.gde.2005.06.012. View

Brennecke J, Hipfner D, Stark A, Russell R, Cohen S . bantam encodes a developmentally regulated microRNA that controls cell proliferation and regulates the proapoptotic gene hid in Drosophila. Cell. 2003; 113(1):25-36. DOI: 10.1016/s0092-8674(03)00231-9. View

Jazdzewski K, Murray E, Franssila K, Jarzab B, Schoenberg D, de la Chapelle A . Common SNP in pre-miR-146a decreases mature miR expression and predisposes to papillary thyroid carcinoma. Proc Natl Acad Sci U S A. 2008; 105(20):7269-74. PMC: 2438239. DOI: 10.1073/pnas.0802682105. View

Loktionov A . Common gene polymorphisms, cancer progression and prognosis. Cancer Lett. 2004; 208(1):1-33. DOI: 10.1016/j.canlet.2004.02.009. View

Kawahara Y, Zinshteyn B, Sethupathy P, Iizasa H, Hatzigeorgiou A, Nishikura K . Redirection of silencing targets by adenosine-to-inosine editing of miRNAs. Science. 2007; 315(5815):1137-40. PMC: 2953418. DOI: 10.1126/science.1138050. View

Hatfield S, Shcherbata H, Fischer K, Nakahara K, Carthew R, Ruohola-Baker H . Stem cell division is regulated by the microRNA pathway. Nature. 2005; 435(7044):974-8. DOI: 10.1038/nature03816. View

Hu Z, Chen J, Tian T, Zhou X, Gu H, Xu L . Genetic variants of miRNA sequences and non-small cell lung cancer survival. J Clin Invest. 2008; 118(7):2600-8. PMC: 2402113. DOI: 10.1172/JCI34934. View

Duan R, Pak C, Jin P . Single nucleotide polymorphism associated with mature miR-125a alters the processing of pri-miRNA. Hum Mol Genet. 2007; 16(9):1124-31. DOI: 10.1093/hmg/ddm062. View

Chen J, Mandel E, Thomson J, Wu Q, Callis T, Hammond S . The role of microRNA-1 and microRNA-133 in skeletal muscle proliferation and differentiation. Nat Genet. 2005; 38(2):228-33. PMC: 2538576. DOI: 10.1038/ng1725. View

10.

Cimmino A, Calin G, Fabbri M, Iorio M, Ferracin M, Shimizu M . miR-15 and miR-16 induce apoptosis by targeting BCL2. Proc Natl Acad Sci U S A. 2005; 102(39):13944-9. PMC: 1236577. DOI: 10.1073/pnas.0506654102. View

11.

Saunders M, Liang H, Li W . Human polymorphism at microRNAs and microRNA target sites. Proc Natl Acad Sci U S A. 2007; 104(9):3300-5. PMC: 1805605. DOI: 10.1073/pnas.0611347104. View

12.

Mao C, Pan H, Chen Q, Wang X, Ye D, Qiu L . Association between Fc receptor-like 3 C169T polymorphism and risk of systemic lupus erythematosus: a meta-analysis. Mol Biol Rep. 2009; 37(1):191-6. DOI: 10.1007/s11033-009-9591-6. View

13.

Tamura Y, Yoshida M, Ohnishi Y, Hohjoh H . Variation of gene silencing involving endogenous microRNA in mammalian cells. Mol Biol Rep. 2008; 36(6):1413-20. DOI: 10.1007/s11033-008-9330-4. View

14.

Dai Y, Sui W, Lan H, Yan Q, Huang H, Huang Y . Comprehensive analysis of microRNA expression patterns in renal biopsies of lupus nephritis patients. Rheumatol Int. 2008; 29(7):749-54. DOI: 10.1007/s00296-008-0758-6. View

15.

Tang Y, Luo X, Cui H, Ni X, Yuan M, Guo Y . MicroRNA-146A contributes to abnormal activation of the type I interferon pathway in human lupus by targeting the key signaling proteins. Arthritis Rheum. 2009; 60(4):1065-75. DOI: 10.1002/art.24436. View

16.

Dai Y, Huang Y, Tang M, Lv T, Hu C, Tan Y . Microarray analysis of microRNA expression in peripheral blood cells of systemic lupus erythematosus patients. Lupus. 2007; 16(12):939-46. DOI: 10.1177/0961203307084158. View

17.

Lafferty-Whyte K, Cairney C, Jamieson N, Oien K, Keith W . Pathway analysis of senescence-associated miRNA targets reveals common processes to different senescence induction mechanisms. Biochim Biophys Acta. 2009; 1792(4):341-52. DOI: 10.1016/j.bbadis.2009.02.003. View

18.

Calin G, Ferracin M, Cimmino A, Di Leva G, Shimizu M, E Wojcik S . A MicroRNA signature associated with prognosis and progression in chronic lymphocytic leukemia. N Engl J Med. 2005; 353(17):1793-801. DOI: 10.1056/NEJMoa050995. View

19.

Wienholds E, Plasterk R . MicroRNA function in animal development. FEBS Lett. 2005; 579(26):5911-22. DOI: 10.1016/j.febslet.2005.07.070. View

20.

Tan E, Cohen A, Fries J, MASI A, McShane D, Rothfield N . The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum. 1982; 25(11):1271-7. DOI: 10.1002/art.1780251101. View