The Antimicrobial Triclocarban Stimulates Embryo Production in the Freshwater Mudsnail Potamopyrgus Antipodarum

Overview

Journal Environ Toxicol Chem

Publisher Oxford University Press

Date 2010 Sep 8

PMID 20821527

Citations 13

Authors

Ben D Giudice

Thomas M Young

Affiliations

Soon will be listed here.

Abstract

Recent research has indicated that the antimicrobial chemical triclocarban (TCC) represents a new type of endocrine disruptor, amplifying the transcriptional activity of steroid hormones and their receptors while itself exhibiting little affinity for these receptors. The effects of TCC were studied in the freshwater mudsnail Potamopyrgus antipodarum. Specimens were exposed to concentrations ranging from 0.05 to 10.5 microg/L dissolved TCC and were removed and dissected, and embryos contained within the brood pouch were counted and classified as shelled or unshelled after two and four weeks of exposure. After four weeks, environmentally relevant TCC concentrations of 1.6 to 10.5 microg/L resulted in statistically significant increases in the number of unshelled embryos, whereas 0.2, 1.6, and 10.5 microg/L exposures significantly increased numbers of shelled embryos. The lowest observed effect concentration (LOEC) was 0.2 microg/L, the no observed effect concentration (NOEC) was 0.05 microg/L; the 10% effective concentration (EC10) and the median effective concentration (EC50) for unshelled effects were 0.5 microg/L and 2.5 microg/L, respectively. Given the widespread occurrence of TCC in the environment and the effects shown at environmentally relevant concentrations, these results indicate that TCC may be causing reproductive effects in the environment. Furthermore, the present study indicates that environmental risk from a new class of endocrine-disrupting chemicals (EDCs) is both qualitatively and quantitatively similar to risk from existing classes of EDCs.

Citing Articles

Antimicrobial Transformation Products in the Aquatic Environment: Global Occurrence, Ecotoxicological Risks, and Potential of Antibiotic Resistance.

Loffler P, Escher B, Baduel C, Virta M, Lai F Environ Sci Technol. 2023; 57(26):9474-9494.

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Triclocarban exposure exaggerates colitis and colon tumorigenesis: roles of gut microbiota involved.

Yang H, Sanidad K, Wang W, Xie M, Gu M, Cao X Gut Microbes. 2019; 12(1):1690364.

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The toxicity of a mixture of two antiseptics, triclosan and triclocarban, on reproduction and growth of the nematode Caenorhabditis elegans.

Vingskes A, Spann N Ecotoxicology. 2018; 27(4):420-429.

PMID: 29411206 DOI: 10.1007/s10646-018-1905-9.

Potential Developmental and Reproductive Impacts of Triclocarban: A Scoping Review.

Rochester J, Bolden A, Pelch K, Kwiatkowski C J Toxicol. 2018; 2017:9679738.

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Maternal exposure to an environmentally relevant dose of triclocarban results in perinatal exposure and potential alterations in offspring development in the mouse model.

Enright H, Falso M, Malfatti M, Lao V, Kuhn E, Hum N PLoS One. 2017; 12(8):e0181996.

PMID: 28792966 PMC: 5549899. DOI: 10.1371/journal.pone.0181996.

References

Duft M, Schulte-Oehlmann U, Tillmann M, Markert B, Oehlmann J . Toxicity of triphenyltin and tributyltin to the freshwater mudsnail Potamopyrgus antipodarum in a new sediment biotest. Environ Toxicol Chem. 2002; 22(1):145-52. View

Bell A . An endocrine disrupter increases growth and risky behavior in threespined stickleback (Gasterosteus aculeatus). Horm Behav. 2004; 45(2):108-14. DOI: 10.1016/j.yhbeh.2003.09.009. View

Guillette Jr L . Endocrine disrupting contaminants--beyond the dogma. Environ Health Perspect. 2006; 114 Suppl 1:9-12. PMC: 1874172. DOI: 10.1289/ehp.8045. View

Kolpin D, Furlong E, Meyer M, Thurman E, Zaugg S, Barber L . Pharmaceuticals, hormones, and other organic wastewater contaminants in U.S. streams, 1999-2000: a national reconnaissance. Environ Sci Technol. 2002; 36(6):1202-11. DOI: 10.1021/es011055j. View

Hayes T, Haston K, Tsui M, Hoang A, Haeffele C, Vonk A . Atrazine-induced hermaphroditism at 0.1 ppb in American leopard frogs (Rana pipiens): laboratory and field evidence. Environ Health Perspect. 2003; 111(4):568-75. PMC: 1241446. DOI: 10.1289/ehp.5932. View

Halden R, Paull D . Co-occurrence of triclocarban and triclosan in U.S. water resources. Environ Sci Technol. 2005; 39(6):1420-6. DOI: 10.1021/es049071e. View

Blanck H, Marcus M, Tolbert P, Rubin C, Henderson A, Hertzberg V . Age at menarche and tanner stage in girls exposed in utero and postnatally to polybrominated biphenyl. Epidemiology. 2000; 11(6):641-7. DOI: 10.1097/00001648-200011000-00005. View

Rayner J, Enoch R, Fenton S . Adverse effects of prenatal exposure to atrazine during a critical period of mammary gland growth. Toxicol Sci. 2005; 87(1):255-66. DOI: 10.1093/toxsci/kfi213. View

Duft M, Schmitt C, Bachmann J, Brandelik C, Schulte-Oehlmann U, Oehlmann J . Prosobranch snails as test organisms for the assessment of endocrine active chemicals--an overview and a guideline proposal for a reproduction test with the freshwater mudsnail Potamopyrgus antipodarum. Ecotoxicology. 2007; 16(1):169-82. DOI: 10.1007/s10646-006-0106-0. View

10.

Chen J, Ahn K, Gee N, Ahmed M, Duleba A, Zhao L . Triclocarban enhances testosterone action: a new type of endocrine disruptor?. Endocrinology. 2007; 149(3):1173-9. PMC: 2275366. DOI: 10.1210/en.2007-1057. View

11.

Matthiessen P . An assessment of endocrine disruption in mollusks and the potential for developing internationally standardized mollusk life cycle test guidelines. Integr Environ Assess Manag. 2008; 4(3):274-84. DOI: 10.1897/IEAM_2008-003.1. View

12.

Mankame T, Hokanson R, Fudge R, Chowdhary R, Busbee D . Alteration of gene expression in human cells treated with the agricultural chemical diazinon: possible interaction in fetal development. Hum Exp Toxicol. 2006; 25(5):225-33. DOI: 10.1191/0960327106ht622oa. View

13.

Hokanson R, Hanneman W, Hennessey M, Donnelly K, McDonald T, Chowdhary R . DEHP, bis(2)-ethylhexyl phthalate, alters gene expression in human cells: possible correlation with initiation of fetal developmental abnormalities. Hum Exp Toxicol. 2007; 25(12):687-95. DOI: 10.1177/0960327106071977. View

14.

Sapkota A, Heidler J, Halden R . Detection of triclocarban and two co-contaminating chlorocarbanilides in US aquatic environments using isotope dilution liquid chromatography tandem mass spectrometry. Environ Res. 2006; 103(1):21-9. DOI: 10.1016/j.envres.2006.03.006. View

15.

Nolen G, Dierckman T . Reproduction and teratogenic studies of a 2:1 mixture of 3,4,4'-trichlorocarbanilide and 3-trifluoromethyl-4,4'-dichlorocarbanilide in rats and rabbits. Toxicol Appl Pharmacol. 1979; 51(3):417-25. DOI: 10.1016/0041-008x(79)90366-1. View

16.

Fenton S . Endocrine-disrupting compounds and mammary gland development: early exposure and later life consequences. Endocrinology. 2006; 147(6 Suppl):S18-24. DOI: 10.1210/en.2005-1131. View

17.

Ahn K, Zhao B, Chen J, Cherednichenko G, Sanmarti E, Denison M . In vitro biologic activities of the antimicrobials triclocarban, its analogs, and triclosan in bioassay screens: receptor-based bioassay screens. Environ Health Perspect. 2008; 116(9):1203-10. PMC: 2535623. DOI: 10.1289/ehp.11200. View

18.

Duft M, Schulte-Oehlmann U, Weltje L, Tillmann M, Oehlmann J . Stimulated embryo production as a parameter of estrogenic exposure via sediments in the freshwater mudsnail Potamopyrgus antipodarum. Aquat Toxicol. 2003; 64(4):437-49. DOI: 10.1016/s0166-445x(03)00102-4. View

19.

Kidd K, Blanchfield P, Mills K, Palace V, Evans R, Lazorchak J . Collapse of a fish population after exposure to a synthetic estrogen. Proc Natl Acad Sci U S A. 2007; 104(21):8897-901. PMC: 1874224. DOI: 10.1073/pnas.0609568104. View