Microarray-based Approach Identifies Differentially Expressed MicroRNAs in Porcine Sexually Immature and Mature Testes

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2010 Sep 1

PMID 20805883

Citations 39

Authors

Lifan Luo

Lianzhi Ye

Gang Liu

Guochao Shao

Rong Zheng

Zhuqing Ren

Bo Zuo

Dequan Xu

Minggang Lei

Siwen Jiang

Changyan Deng

Yuanzhu Xiong

Fenge Li

Affiliations

Soon will be listed here.

Abstract

Background: MicroRNAs (miRNAs) are short non-coding RNA molecules which are proved to be involved in mammalian spermatogenesis. Their expression and function in the porcine germ cells are not fully understood.

Methodology: We employed a miRNA microarray containing 1260 unique miRNA probes to evaluate the miRNA expression patterns between sexually immature (60-day) and mature (180-day) pig testes. One hundred and twenty nine miRNAs representing 164 reporter miRNAs were expressed differently (p<0.1). Fifty one miRNAs were significantly up-regulated and 78 miRNAs were down-regulated in mature testes. Nine of these differentially expressed miRNAs were validated using quantitative RT-PCR assay. Totally 15,919 putative miRNA-target sites were detected by using RNA22 method to align 445 NCBI pig cDNA sequences with these 129 differentially expressed miRNAs, and seven putative target genes involved in spermatogenesis including DAZL, RNF4 gene were simply confirmed by quantitative RT-PCR.

Conclusions: Overall, the results of this study indicated specific miRNAs expression in porcine testes and suggested that miRNAs had a role in regulating spermatogenesis.

Citing Articles

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