Polybacterial Challenge Effects on Cytokine/chemokine Production by Macrophages and Dendritic Cells

Overview

Journal Inflamm Res

Specialties Allergy & Immunology
Pathology

Date 2010 Aug 28

PMID 20798974

Citations 9

Authors

C B Huang

Y Altimova

S Strange

J L Ebersole

Affiliations

Soon will be listed here.

Abstract

Objective: To investigate the polymicrobial infection of periodontal disease, which elicits inflammatory mediators/cytokines/chemokines in the local gingival tissues, and a polybacterial challenge of antigen-presenting cells, e.g. macrophages and dendritic cells (DCs), at the mucosal surface.

Materials And Methods: The cytokine/chemokine profiles of human macrophages and DCs in response to polybacterial challenges were investigated.

Results: Oral Gram-negative bacteria elicited significantly greater IL-8 levels from macrophages, compared to Gram-positive bacteria. Gram-positive bacteria did not show synergism in inducing this chemokine from macrophages. In contrast, pairs of oral Gram-negative bacteria elicited synergistic production of IL-8 by macrophages. Similar results were not observed with TNFα, which only appeared additive with the polybacterial challenge. Selected Gram-negative bacterial pairs synergized in IL-6 production by immature DCs. In mature DCs (mDCs), a Porphyromonas gingivalis/Fusobacterium nucleatum and Porphyromonas intermedia/F. nucleatum polybacterial challenge resulted in significant synergism for IL-6 and TNFα levels. However, only the Pi/Fn combination synergized for IL-12 production and there appeared to be no polybacterial effect on IL-10 production by the mDCs.

Conclusions: These results indicate that a polybacterial challenge of cells linking innate and adaptive immune responses results in varied response profiles that are dependent upon the characteristics of the microorganisms that are components of the polybacterial complex.

Citing Articles

Sialidase Deficiency in Increases IL-12 Secretion in Stimulated Macrophages Through Regulation of CR3, IncRNA GAS5 and miR-21.

Yang X, Pan Y, Xu X, Tong T, Yu S, Zhao Y Front Cell Infect Microbiol. 2018; 8:100.

PMID: 29675399 PMC: 5895773. DOI: 10.3389/fcimb.2018.00100.

Macrophage polarization in response to oral commensals and pathogens.

Huang C, Alimova Y, Ebersole J Pathog Dis. 2016; 74(3).

PMID: 26884502 PMC: 5975235. DOI: 10.1093/femspd/ftw011.

Immuno-pathogenesis of Periodontal Disease: Current and Emerging Paradigms.

Huang N, Gibson 3rd F Curr Oral Health Rep. 2014; 1(2):124-132.

PMID: 24839590 PMC: 4020182. DOI: 10.1007/s40496-014-0017-8.

Pro-inflammatory, Th1, Th2, Th17 cytokines and dendritic cells: a cross-sectional study in chronic periodontitis.

Souto G, Queiroz-Junior C, de Abreu M, Costa F, Mesquita R PLoS One. 2014; 9(3):e91636.

PMID: 24670840 PMC: 3966767. DOI: 10.1371/journal.pone.0091636.

Periodontal disease immunology: 'double indemnity' in protecting the host.

Ebersole J, Dawson 3rd D, Morford L, Peyyala R, Miller C, Gonzalez O Periodontol 2000. 2013; 62(1):163-202.

PMID: 23574466 PMC: 4131201. DOI: 10.1111/prd.12005.

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